Thermo Scientific™ OmniPac® PCX-500 Mixed-Mode Column by Thermo Fisher Scientific

The multimode separation mechanism is achieved by coating a macroporous hydrophobic core for reversed-phase retention with a cation-exchange latex bead layer for cation-exchange retention. The macroporous structure provides an accessible hydrophobic core where reversed-phase retention occurs. The cation-exchange selectivity is provided by a cation-exchange MicroBead™ layer that coats the hydrophobic core. Since the cation-exchange layer is identical to that of the OmniPac PCX-100, the two columns exhibit similar ion-exchange selectivity.

As a result, the OmniPac PXC-100 can be used to develop the ion-exchange separation, and the method can then be transferred to the OmniPac PXC-500 for further refinement to include solvent for the reversed-phase separation. The OmniPac PCX-500 is 100% solvent compatible and should always be run with at least 1% organic solvent in the mobile phase to maintain column life.


OmniPac® PCX-500 Mixed-Mode Column Features:

• Good for the simultaneous separation of neutral and cationic species
• Acid and base compatible, pH 0–14
• 100% solvent compatible
• Not as effective as the CS12A for inorganic cations

Column Details:

• Length: 250 mm
• ID: 4.0 mm