Odyssey® Fc Imaging System by LI-COR Biosciences

"The instrument is safe and relatively simple to operate. It has helped us save on reagents and time. It is primarily operated by our undergraduate students and research students, and requires minimal training. It is a very versatile instrument for a primarily-undergraduate research program."

"NIR western blotting provides superior detection when compared with traditional chemiluminescent techniques. To this end, we migrated into the LI-COR workflow two years ago and haven't looked back. Their product allows you to keep your existing western blotting equipment, and with relatively minor adjustments (changing the secondary antibody, namely), your workflow becomes NIR. Their scanner is easy to use, provides clear images, and their accompanying software makes analysis & documentation easy."

"Easy to use machine that allows the quick visualization of multiplexed membranes, could not go back to the hassle of ECL use and membrane stripping with chemiluminescent Western blotting!"

"Excellent results over a wide dynamic range. Easy to use as a shared instrument (many users). Can multiplex which saves time and money."

"High quality results. Easy to use. No x-ray film required. Saves time, money and the environment. Highly recommend to others"

"High resolution scanned image. High sensitivity to detect western blot bands. Easy to use. Save time. No dark room and x-films needed."

"Borrowed from another lab. This thing is terrific. I want one!"

"The Odyssey Fc has been an awesome addition to the lab. The machine allows for multiplexing of antibodies which is extremely useful. Our local technical rep has been an extremely valuable resource as well."

"Awesome product, easy to use! Friendly interface, good customer care"

"With the Odyssey® Fc Imaging System, we are now able to determine our mutation specific knock down very precisely and highly reproducible on one blot. The sensitivity of the fluorescent labeled secondary antibodies is outstanding."

"It is definitely providing a high quality result when compared to older systems. The software is user friendly and can be easily customized."

"This instrument is extremely easy to use and produces results very quickly. The visual results can be hit-or-miss. Sometimes the image is of impeccable quality, very high resolution, and other times it is grainy and unfocused. I don't know enough about how the image is captured to correct this. Overall I would recommend this product for visualizing Westerns."

"I love this instrument - super easy to use for ECL or fluorescence. The data can be easily quantified and images adjusted. Everything is also in one place - data with exposure times (no more files of film). We also received excellent training and the support, whether for reagents or troubleshooting, is also great."

"Everyone using the instrument has found it very easy to use. It has great sensitivity and the ability to multiplex antibodies has been great. The support reps have been very responsive to questions and troubleshooting."

"Excellent piece of equipment. Easy to use, intuitive interface, and gives high-quality images. Quantification of bands is simple and accurate."

"Super high quality results on Westerns. The images blow away ECL-film or HRP/AP color development on nitrocellulose. Directly compared to other (non-IR dye) competitors for linearity of signal detection. Odyssey was the only one that came close to accurately measuring 10-fold serial dilutions of a protein sample over 3 orders of magnitude. Quantitation is believable with this machine & IR dyes. IR dyes have very low background. Sloppy undergrads get beautiful results as long as they process the samples per protocol (stringy DNA will ruin any gel run) and clean the equipment after use (MeOH washes for the transfer sponges are important to do regularly). This machine should be a workhorse for any lab that's doing westerns on a daily basis. Best money I've ever spent on equipment. "

"I have used Odyssey products for 8 years and I have never had a problem with the equipment or the reagents from Li-Cor. Everything is of the highest quality and has made quantitative Western blot detection extremely easy and reliable. The equipment is very robust and I have yet to have a need for service. I can highly recommend the Odyssey Fc. "

"Simple and straightforward to use. LI-COR offer good customer service and technical support. "

"The Li-Cor Odyssey Fc Imaging System is easy to use and the operation is self-explanatory. Two different wavelengths for western blot fluorescent dyes (680 and 800nm) and PCR gels dyed with gel red (detection 600 nm) are available. Gels and blots can be detected for 30 seconds, 2 minutes or 10 minutes. Quantitative analysis of the spots is fast and easy. Pictures can be exported as .jpeg .tiff or PNG with 150, 300 or 600dpi. The only negative is that the handling of the software could be based more on windows-handling (e.g. shortcuts)."

"Love not having to waste film and able to adjust exposure."

"It is easy to use and good value for money. We also liked the service after sale. We would absolutely recommend it to all researchers in this field."

"The Odyssey Fc Imaging Station has worked really well for our group. It is easy to use and allows us to image our blots quickly. Although it took us a little while to figure out the best (and cheapest) blocking solution for our purposes, our blots look great and the IR dyes seem quite sensitive. We've even had instances where the IR dyes have been more sensitive than traditional ECL and film. We also really enjoy the ease of quantifying our bands with the Odyssey software. Overall, this was a great purchase for our lab, which routinely uses immunoblotting in our work. I would highly recommend this instrument to anyone looking to more accurately quantify their Westerns or streamline their Western blotting."