Odyssey® Blocking Buffer (TBS) by LI-COR Biosciences
Status:
Reviewer
Member since: 2015
Organization: Rutgers University
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- Ease of use
- After sales service
- Value for money
LiCor Blocking buffer. Greatly enhanced my Western detection abilities.
Application Area: Western analysis
"I recently found that using the LiCor Odyssey Blocking Buffer (PBS) with 0.1% Tween in both my primary and secondary antibody incubation steps helps to both reduce background and to enhance the primary target. This finding is in comparison with performing both incubations in PBS Tween alone. The added benefit is that following the initial blocking in the LiCor Blocking Buffer, one can advance to the primary incubation directly- skipping over the PBS Tween washing steps that take 15-30 minutes. Results likely vary with primary and secondary antibodies but this helped me greatly using a Roche AntiGFP primary antibody and the IR800 secondary antibody from LiCor."
Review date: 02 Feb 2017 | Odyssey® Blocking Buffer (TBS)
Status:
Reviewer Member since: 2016
Organization: The Scripps Research Institute
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- Ease of use
- After sales service
- Value for money
Consistent, dependable results.
Application Area:Western Blotting
"Easy, quick and generally very good blocking of blots. A bit pricey, so you want to re-use blocker to prepare antibodies."
Review date: 27 Sep 2016 | Odyssey® Blocking Buffer (TBS)
Status:
Reviewer Member since: 2015
Organization: SUNY Upstate Medical University
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- Ease of use
- After sales service
- Value for money
Western blots using these blocking buffers look great.
Application Area:Cortical tissue protein analysis
"This product really helps to eliminate background signal. It can be used by itself or as a 50/50 TBS mixture which helps to reduce costs."
Review date: 22 Sep 2016 | Odyssey® Blocking Buffer (TBS)
Status:
Reviewer Member since: 2016
Organization: Saint Mary's University of Minneosta
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- Ease of use
- After sales service
- Value for money
Great product. Wish I knew their secret!!!
Application Area:Blocking membranes for Western Blots
"I have tried several other blocking products and our own "homemade" solutions and in side-by-side comparisons, the Odyssey Blocking Buffer (we used PBS) has always given us the cleanest background and most intense signal. It is a bit pricey but results are great."
Review date: 22 Sep 2016 | Odyssey® Blocking Buffer (TBS)
Status:
Reviewer Member since: 2016
Organization: GA-PCOM, Division of Research
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- Ease of use
- After sales service
- Value for money
Great product, do not want to look for a replacement.
Application Area:Use for blocking the blot, and use it to make both primary and secondary antibody diluent for wester
"The LI-COR blocking buffer is very easy to use for highly reproducible results. Whenever we have question or need help, the service group are always very help. In terms of value for money, it is not that cheap, however it is worth every penny after we compare all the cheaper alternatives. "
Review date: 22 Sep 2016 | Odyssey® Blocking Buffer (TBS)
Status:
Reviewer Member since: 2016
Organization: CSU
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- Ease of use
- After sales service
- Value for money
Awesome blocking buffer
Application Area:Western blot
"These blocking buffers work great especially in combination with the secondary antibodies and imaging through Licor."
Review date: 22 Sep 2016 | Odyssey® Blocking Buffer (TBS)
Status:
Reviewer Member since: 2016
Organization: NYMC
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- Ease of use
- After sales service
- Value for money
Very effective blocking buffer
Application Area:Analyze protein expression in tissue and cell culture samples
"Our lab has found this to be a very good blocking buffer. Side by side, blots are much cleaner vs. BSA or milk."
Review date: 19 Sep 2016 | Odyssey® Blocking Buffer (TBS)
Status:
Reviewer Member since: 2016
Organization: University of Pittsburgh
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- Ease of use
- After sales service
- Value for money
I love this blocking buffer, it works every time.
Application Area:Western Blot
"I use Odyssey Blocking Buffer exclusively for all of my western blots. It is much better than using milk for blocking. I never have any problems with background issues and the blots results are clear. It is extremely easy to use and I use it full strength for the blocking step and also for antibody dilutions."
Review date: 19 Sep 2016 | Odyssey® Blocking Buffer (TBS)
Status:
Advanced Reviewer Member since: 2010
Organization: TEMPLE UNIV.
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- Ease of use
- After sales service
- Value for money
Great product
Application Area:Western blotting
"The product is great. Gives really good results for western blots without background unlike milk."
Review date: 16 Dec 2015 | Odyssey® Blocking Buffer (TBS)
Status:
Advanced Reviewer Member since: 2014
Organization: Universidade Federal de Minas Gerais
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- Ease of use
- After sales service
- Value for money
Application Area:membrane blocking for western blot
"Odyssey® Blocking Buffers is really simple and easy to use. It can really block nitrocellulose or PVDF membranes against non-specific binding. However, I only used it for Odyssey scanner system. This product can be reused several times, but you have to take care keeping it at 4ºC. This buffer is really good but the price is not so good when compared with dry milk, for instance, when the role of both is basically the same. The fact you can reuse it and sometimes some antibodies are not so good and you really need a higher quality product for blocking, this buffer is recommended."
Review date: 08 Dec 2014 | Odyssey® Blocking Buffer (TBS)
Odyssey Blocking Buffers are the gold standard for quantitative Western blot detection. They contain no mammalian proteins, for the lowest cross-reactivity with mammalian antibodies and are available in a ready-to-use formulation in TBS (Tris-buffered saline). The TBS-based buffer is ideal for the detection of phosphorylated targets and is suitable for the widest range of targets for Western blot detection. Be sure to optimize your experimental conditions for the best Western blot and assay results. Blocker choice is important for immunoassay success. Blocking buffers enhance sensitivity by reducing background interference, increasing signal-to-noise ratio, and promoting specific binding of the primary antibody while minimizing non-specific interactions.
Applications of the Odyssey Blocking Buffers:
- Western blots In-Cell Western™ assays
- Protein arrays.
Need to learn the basics of Western blotting? Sign up for on-demand Western blot training at Lambda U (www.lambdau.net).
Product Overview
Odyssey® Blocking Buffer (TBS)
Manufacturer LI-COR Biosciences | Available Worldwide
4.5 / 5.0 | 10 reviews
