Odyssey® Blocking Buffers

Odyssey Blocking Buffers are the gold standard for quantitative Western blot detection.They contain no mammalian proteins, for the lowest cross-reactivity with mammalian antibodies and are available in a ready-to-use formulation in PBS (phosphate-buffered saline) or TBS (Tris-buffered saline). The PBS-based buffer is an excellent choice for biotin-streptavidin detection. The TBS-based buffer is ideal for the detection of phosphorylated targets and is suitable for the widest range of targets for Western blot detection. Be sure to optimize your experimental conditions for the best Western blot and assay results. Blocker choice is important for immunoassay success. Blocking buffers enhance sensitivity by reducing background interference, increasing signal-to-noise ratio, and promoting specif...Read more

Receive your quote directly from LI-COR Biosciences.

Reviews

1 Scientist has reviewed this product


Write your own review

Average Rating: 4.0

Ease of use
5 out of 5
After sales service
4 out of 5
Value for money
3 out of 5
Rating: 4.0
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money
3 out of 5
  • Review date: 08 Dec 2014

  • Application Area: membrane blocking for western blot

  • Odyssey® Blocking Buffers

"Odyssey® Blocking Buffers is really simple and easy to use. It can really block nitrocellulose or PVDF membranes against non-specific binding. However, I only used it for Odyssey scanner system. This product can be reused several times, but you have to take care keeping it at 4ºC. This buffer is really good but the price is not so good when compared with dry milk, for instance, when the role of both is basically the same. The fact you can reuse it and sometimes some antibodies are not so good and you really need a higher quality product for blocking, this buffer is recommended."

Description

Odyssey Blocking Buffers are the gold standard for quantitative Western blot detection.

They contain no mammalian proteins, for the lowest cross-reactivity with mammalian antibodies and are available in a ready-to-use formulation in PBS (phosphate-buffered saline) or TBS (Tris-buffered saline). The PBS-based buffer is an excellent choice for biotin-streptavidin detection. The TBS-based buffer is ideal for the detection of phosphorylated targets and is suitable for the widest range of targets for Western blot detection. Be sure to optimize your experimental conditions for the best Western blot and assay results. Blocker choice is important for immunoassay success. Blocking buffers enhance sensitivity by reducing background interference, increasing signal-to-noise ratio, and promoting specific binding of the primary antibody while minimizing non-specific interactions.

Applications of the Odyssey Blocking Buffers:
  • Western blots
  • In-Cell Western™ assays
  • Protein arrays

Product Overview

Product Image

Odyssey® Blocking Buffers

by LI-COR Biosciences

4.0/5.0 (1 reviews)
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money
3 out of 5






Receive your quote directly from LI-COR Biosciences for Odyssey® Blocking Buffers