Odyssey® Blocking Buffers

Odyssey® Blocking Buffers
by LI-COR Biosciences
 4.0/5.0 (1 review)

Odyssey Blocking Buffers are the gold standard for quantitative Western blot detection. They contain no mammalian proteins, for the lowest cross-reactivity with mammalian antibodies and are available in a ready-to-use formulation in PBS (phosphate-buffered saline) or TBS (Tris-buffered saline). The PBS-based buffer is an excellent choice for biotin-streptavidin detection. The TBS-based buffer i...read more

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Reviews

AVERAGE RATING: 4.0

1 scientist has reviewed this product

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  • 5 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 3 out of 5VALUE FOR MONEY

Antonio Peluso


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Member since 2014

Job Title:
PhD Student
Discipline:
Biotechnology
Work Field:
Academia / University
Organization:
Universidade Federal de Minas Gerais
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RATING: 4.0

  • 5 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 3 out of 5VALUE FOR MONEY




Review date: 08 Dec 2014

Application Area: membrane blocking for western blot
Odyssey® Blocking Buffers

"Odyssey® Blocking Buffers is really simple and easy to use. It can really block nitrocellulose or PVDF membranes against non-specific binding. However, I only used it for Odyssey scanner system. This product can be reused several times, but you have to take care keeping it at 4ºC. This buffer is really good but the price is not so good when compared with dry milk, for instance, when the role of both is basically the same. The fact you can reuse it and sometimes some antibodies are not so good and you really need a higher quality product for blocking, this buffer is recommended."

Description

 

Odyssey Blocking Buffers are the gold standard for quantitative Western blot detection.

They contain no mammalian proteins, for the lowest cross-reactivity with mammalian antibodies and are available in a ready-to-use formulation in PBS (phosphate-buffered saline) or TBS (Tris-buffered saline). The PBS-based buffer is an excellent choice for biotin-streptavidin detection. The TBS-based buffer is ideal for the detection of phosphorylated targets and is suitable for the widest range of targets for Western blot detection. Be sure to optimize your experimental conditions for the best Western blot and assay results. Blocker choice is important for immunoassay success. Blocking buffers enhance sensitivity by reducing background interference, increasing signal-to-noise ratio, and promoting specific binding of the primary antibody while minimizing non-specific interactions.

Applications of the Odyssey Blocking Buffers:

  • Western blots
  • In-Cell Western™ assays
  • Protein arrays

Product Overview

Odyssey® Blocking Buffers by LI-COR Biosciences
Odyssey® Blocking Buffers




  • 5out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 3 out of 5VALUE FOR MONEY





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