Odyssey® Blocking Buffers

Manufacturer LI-COR Biosciences  |  Available Worldwide
4.6
/
5.0
  |  8 reviews


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Average Rating: 4.6
8 Scientists have reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money


Western blots using these blocking buffers look great.
Rating: 5.0

  • Application Area: Cortical tissue protein analysis

"This product really helps to eliminate background signal. It can be used by itself or as a 50/50 TBS mixture which helps to reduce costs."

Review date: 22 Sep 2016 | Odyssey® Blocking Buffers
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Saint Mary's University of Minneosta



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Great product. Wish I knew their secret!!!
Rating: 4.7

  • Application Area:Blocking membranes for Western Blots

"I have tried several other blocking products and our own "homemade" solutions and in side-by-side comparisons, the Odyssey Blocking Buffer (we used PBS) has always given us the cleanest background and most intense signal. It is a bit pricey but results are great."

Review date: 22 Sep 2016 | Odyssey® Blocking Buffers
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: GA-PCOM, Division of Research



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Great product, do not want to look for a replacement.
Rating: 4.7

  • Application Area:Use for blocking the blot, and use it to make both primary and secondary antibody diluent for wester

"The LI-COR blocking buffer is very easy to use for highly reproducible results. Whenever we have question or need help, the service group are always very help. In terms of value for money, it is not that cheap, however it is worth every penny after we compare all the cheaper alternatives. "

Review date: 22 Sep 2016 | Odyssey® Blocking Buffers
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: CSU



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Awesome blocking buffer
Rating: 5.0

  • Application Area:Western blot

"These blocking buffers work great especially in combination with the secondary antibodies and imaging through Licor."

Review date: 22 Sep 2016 | Odyssey® Blocking Buffers
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: NYMC



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Very effective blocking buffer
Rating: 4.7

  • Application Area:Analyze protein expression in tissue and cell culture samples

"Our lab has found this to be a very good blocking buffer. Side by side, blots are much cleaner vs. BSA or milk."

Review date: 19 Sep 2016 | Odyssey® Blocking Buffers


  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Pittsburgh



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    3 out of 5
I love this blocking buffer, it works every time.
Rating: 3.7

  • Application Area:Western Blot

"I use Odyssey Blocking Buffer exclusively for all of my western blots. It is much better than using milk for blocking. I never have any problems with background issues and the blots results are clear. It is extremely easy to use and I use it full strength for the blocking step and also for antibody dilutions."

Review date: 19 Sep 2016 | Odyssey® Blocking Buffers
  • Status:

    Advanced Reviewer

  • Member since: 2010

  • Organization: TEMPLE UNIV.



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great product
Rating: 5.0

  • Application Area:Western blotting

"The product is great. Gives really good results for western blots without background unlike milk."

Review date: 16 Dec 2015 | Odyssey® Blocking Buffers
  • Status:

    Advanced Reviewer

  • Member since: 2014

  • Organization: Universidade Federal de Minas Gerais



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    3 out of 5
Rating: 4.0

  • Application Area:membrane blocking for western blot

"Odyssey® Blocking Buffers is really simple and easy to use. It can really block nitrocellulose or PVDF membranes against non-specific binding. However, I only used it for Odyssey scanner system. This product can be reused several times, but you have to take care keeping it at 4ºC. This buffer is really good but the price is not so good when compared with dry milk, for instance, when the role of both is basically the same. The fact you can reuse it and sometimes some antibodies are not so good and you really need a higher quality product for blocking, this buffer is recommended."

Review date: 08 Dec 2014 | Odyssey® Blocking Buffers
Odyssey Blocking Buffers are the gold standard for quantitative Western blot detection.

They contain no mammalian proteins, for the lowest cross-reactivity with mammalian antibodies and are available in a ready-to-use formulation in PBS (phosphate-buffered saline) or TBS (Tris-buffered saline). The PBS-based buffer is an excellent choice for biotin-streptavidin detection. The TBS-based buffer is ideal for the detection of phosphorylated targets and is suitable for the widest range of targets for Western blot detection. Be sure to optimize your experimental conditions for the best Western blot and assay results. Blocker choice is important for immunoassay success. Blocking buffers enhance sensitivity by reducing background interference, increasing signal-to-noise ratio, and promoting specific binding of the primary antibody while minimizing non-specific interactions.

Applications of the Odyssey Blocking Buffers:
  • Western blots
  • In-Cell Western™ assays
  • Protein arrays

Product Overview

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Odyssey® Blocking Buffers

Manufacturer LI-COR Biosciences  |  Available Worldwide

4.6 / 5.0 | 8 reviews