The NucleoCounter offers unique ease of use and reliability for the determination of cell concentration in a sample of cultured mammalian cells. The instrument requires no initialisation or priming, and is ready to use within seconds. NucleoCounter is a compact instrument, which fits perfectly in any cell laboratory and the size and robustness makes it easy to move from one location to another.The sample preparation involves the mixing of sample and lysing buffer, which effectively makes the cells membrane permeable to the DNA staining dye in few seconds, but also is extremely effective in the dispersion of cell aggregates.Omitting the sample preparation allows for the determination of viability using the method of propidium iodide exclusion. The DNA staining dye will be excluded from via...Read more
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