To meet the needs of researchers in the preparation of, for example, plasmid or genomic DNA, Tecan offers a number of automated systems of different sizes, scale and capacity.
These systems allow for the purification of nucleic acid in sufficient quantity and quality for subsequent use in amplification or analytical steps. Tecan provides a range of systems to meet the different needs in terms of scale for the end user. These range from smaller-scale solutions based on the MiniPrep series of liquid handlers (60- or 75-cm deck size) and larger nucleic acid preparation workstations all the way up to the fully automated TRAC (Tecan Robotic Assay Composer) systems for high throughput applications.
These systems fully support the different purification procedures needed for different types of nucleic acid samples. For example, Tecan systems may include options for vacuum/solid phase extraction (SPE) modules (Te-VacS), magnetic bead separation modules (Te-MagS) or the integration of a centrifuge (Centrifuge option on the Genesis Freedom). Tecan’s liquid handling platforms are fully capable of handling tube-to-plate transfers and the many aqueous and organic solvents typically used in nucleic acid extraction procedures. Different types of commercially available kits for nucleic acid preparation may be integrated into these automated systems including those from many leading suppliers.
Quantitation is an essential component of all molecular biology procedures. Accordingly, Tecan offers a wide variety of detection devices for nucleic acid quantitation. For ultraviolet absorbance quantitation and testing purity, Tecan provides GENios plate reader. this instrument offers advanced fluorescent measurement possibilities for nucleic acid measurements and are easily integrated into the different liquid handling systems available from Tecan.
As for the nucleic acid sample preparation suite, Tecan provides a full range of systems to support automated PCR and sequencing reactions. These integrated solutions range from the smaller MiniPrep systems through to workstations and the larger TRAC-based fully automated systems. To meet the many requirements of researchers for PCR, Tecan has assembled systems to support PCR sample preparation, the PCR reaction itself, and the subsequent cleanup of PCR products. For DNA sequencing reactions, Tecan systems are able to accurately handle all the necessary reagents and probes required for typical sequencing reactions (for example, dideoxynucleotide sequencing or chain termination methods, Maxam & Gilbert).
By delivering reliability, flexibility and quality of results, Tecan’s automated solutions for nucleic acid sample preparation, PCR and sequencing are capable of performing most of the diverse molecular biology procedures needed to work with nucleic acids.