Nitrotetrazolium Blue chloride by MilliporeSigma
NBT is prepared synthetically. The most common application for NBT is the detection of alkaline phosphatase on western blots. NBT has also been used as a redox indicator for other enzymatic reactions including dehydrogenases, threonine deaminase, glucose-6-phosphate dehydrogenase, phosphofructokinase on polyacrylamide gels, oxidases on polyacrylamide gels, and pentose shunt dehydrogenses.Redox and halfwave potentials have been determined for NBT. NBT has also been used as a colorimetric in...
Read more