Nitrotetrazolium Blue chloride by MilliporeSigma
Manufacturer MilliporeSigma
Nitrotetrazolium Blue chloride
NBT is prepared synthetically. The most common application for NBT is the detection of alkaline
phosphatase on western blots. NBT has also been used as a redox indicator for other enzymatic reactions including dehydrogenases, threonine deaminase, glucose-6-phosphate dehydrogenase, phosphofructokinase on polyacrylamide gels, oxidases on polyacrylamide gels, and pentose shunt
dehydrogenses.
Redox and halfwave potentials have been determined for NBT. NBT has also been used as a colorimetric indicator of bacterial infection in blood samples.
phosphatase on western blots. NBT has also been used as a redox indicator for other enzymatic reactions including dehydrogenases, threonine deaminase, glucose-6-phosphate dehydrogenase, phosphofructokinase on polyacrylamide gels, oxidases on polyacrylamide gels, and pentose shunt
dehydrogenses.
Redox and halfwave potentials have been determined for NBT. NBT has also been used as a colorimetric indicator of bacterial infection in blood samples.
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