Ni Sepharose excel affinity media for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC). • Load eukaryotic cell culture samples containing secreted histidine-tagged proteins directly with retained binding capacity • Increase target protein yield and decrease degradation through reduced and simplified sample handling • Choose between several formats for screening and preparative purification of histidine-tagged proteins Ni Sepharose excel is an IMAC medium precharged with nickel ions very strongly bound to a chelating ligand. Samples that usually cause stripping of metal ions can therefore be loaded to the medium. The nickel ions have been shown...Read more
Receive your quote directly from GE Healthcare.