Purification of HisTag fusion proteins by metal chelation chromatography
The HisBind® family of products offers a wide selection of supports designed for rapid one-step purification of proteins containing the HisTag® sequence by immobilized metal affinity chromatography (IMAC).
The HisTag sequence (6, 8 or 10 consecutive histidine residues) binds to divalent cations (Ni2+) immobilized on NTA*- and IDA-based HisBind resins. After unbound proteins are washed away, the target protein is recovered by elution with either imidazole or slight reduction in pH. This versatile system enables proteins to be purified under gentle, non-denaturing conditions, or in the presence of either 6 M guanidine or urea. Further details can be found in the HisBind Purification Kits Overview. *manufactured by QIAGEN
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