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NGC Scout 10 Plus Chromatography System by Bio-Rad
Status:
Reviewer
Member since: 2008
Organization: NIBSC
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- Ease of use
- After sales service
- Value for money
Brilliant bit of kit that doesn't fail on large scale purification.
Application Area: Recombinant protein purification
"The NGC is a modular HPLC from Bio-Rad. We have 2 such systems in the institute. Recently our detection module bulb went just before an HPLC run. We were able to swap out a module from another machine and we had the machine up and running in 20 minutes. Contacting Bio-Rad, we were told what the fault could be and directed to a video on how to fix the problem – in our case a bulb change. We were also able to order the bulb which was promptly delivered. Changing was easy too. Although it is a learning curve to do your own maintenance, the useful videos and guides provided by Bio-Rad saves on the labor costs and time taken to arrange for a service engineer. Free software and firmware upgrades and multiple licenses make this machine truly flexible. "
Review date: 30 Oct 2020 | NGC Scout 10 Plus Chromatography System
The NGC™ Scout 10 Plus Chromatography System is designed for medium throughput labs. It has automated 10 ml/min pumps that provide accurate gradients for high-resolution separations for any application. This instrument may be used for creating automated buffer gradients with buffer blending for rapid scouting and purification of proteins, peptides, and nucleic acids.
The NGC Scout 10 Plus System has a multi-wavelength detector with simultaneous four-wavelength monitoring for high-accuracy detection of proteins, peptides, and nucleic acids combined with conductivity measurements. The system also allows gradient separations at different pH values for rapid method development. Fractionated samples can be easily collected from analytical- to preparative-scale purifications using the NGC Fraction Collector and BioFrac™ Fraction Collector .
To learn more, watch the NGC System tour.
The NGC Scout 10 Plus System includes:
- 1 NGC Two-Tier Base Frame
- 1 NGC F10 Pump Module for a flow rate of 0.001–10 ml/min at 3,650 psi (252 bar, 25.2 MPa)
- 1 NGC Mixer Module , small (263 µl); includes an extension mixer barrel (750 µl)
- 1 NGC Multi-Wavelength Detector Module for simultaneous four-wavelength detection at 190–800 nm and salt gradient monitoring, with integrated conductivity monitor; includes tubing and fittings and one 5 mm flow cell
- 1 NGC Sample Injection Valve Kit for automated application of small sample volumes from sample loops or large sample volumes using an optional sample pump
- 1 NGC Buffer Blending Valve Module
- 1 NGC pH Valve Module , includes 1 NGC pH Probe (Ag/AgCl)
- 1 integrated system touch screen
- 1 copy of ChromLab™ Software for instrument control, data collection, and data analysis
- 1 documentation CD containing manuals for use with NGC systems
- 1 NGC Buffer Tray
- 1 NGC Communication Adaptor for connecting the BioFrac Fraction Collector
Features and Benefits of the NGC Scout 10 Plus Chromatography System
A flexible, customizable chromatography system to suit both your application and workflow needs:
- The Guided Fluidics selection tool (patent pending) coupled with the Module Discovery system enables a single-click application-based system setup through automated module function recognition
- The Tier Rotate™ design allows optimal placement of valves and detectors to minimize the flow path
- Compact footprint – fits in a refrigerator
- Point-to-Plumb™ lighting shows the step-by-step LED-guided plumbing of the system
- Unique ChromLab software provides powerful system control, automated methods, and analysis options
Applications and Uses of Medium-Pressure Chromatography Systems
Preparative- to analytical-scale isolation, purification, and analysis of multiple types of molecules:
- Recombinant protein purification and refolding
- Monoclonal antibody purification
- Removal of viruses, protein preparations
- Analysis of plasma proteins for disease diagnosis
More Information
NGC System modular components allow your chromatography system to meet both laboratory space and application requirements. As these requirements change, systems may be easily reconfigured and seamlessly upgraded with increased functionality, such as higher flow rates , more sophisticated detection capabilities , pH monitoring , column scouting , and buffer blending . Use the configurator tool to build the best system to fit your needs.
A PC controller enables easy communication with the workstation and peripheral devices. The controller includes the Windows 7 operating system, application software, a keyboard, mouse, and high-resolution flat-panel monitor.
