NGC Quest 100 Chromatography System by Bio-Rad

• Automated 100 ml/min pumps provide accurate buffer gradients for high-resolution separation of biomolecules
• Automated sample injection using fixed or dynamic loops
• Gradient separations at different pH values using the mixer module, for rapid method development
• High-accuracy detection of proteins with simultaneous conductivity measurements
• Compatibility with the C-96 Autosampler , available with or without cooling, for accurate and reproducible sample injections
• Compatibility with the NGC Fraction Collector and BioFrac™ Fraction Collector for sample collection

The NGC Quest 100 System includes the following modules and kits:

• 1 NGC Two-Tier Base Frame
• 2 NGC F100 Pump Modules , for generating a flow rate of 0.01–100 ml/min at 1,450 psi (100 bar, 10 MPa)
• 1 NGC Mixer Module and 750 µl base and top assembly , includes an extension mixer barrel (5 ml)
• 1 NGC Single-Wavelength Detector Module — UV detection at 255 or 280 nm combined with conductivity monitor, includes tubing and fittings and one 5 mm flow cell
• 1 NGC Sample Inject Valve Module for automated application of small sample volumes from sample loops or large sample volumes using an optional sample pump
• 1 integrated system touch screen
• 1 copy of ChromLab™ Software for instrument control, data collection, and data analysis
• 1 documentation DVD containing manuals for use with NGC Systems
• 2 NGC Blank Modules
• 1 NGC Buffer Tray
• 1 NGC Communication Adaptor for connecting the BioFrac Fraction Collector

Applications and Uses of Medium-Pressure Chromatography Systems

• Preparative chromatography
• Protein purification and isolation

Related Products

The NGC Quest™ 100 Plus System provides all the capabilities of the NGC Quest 100 System and features a multi-wavelength detector. All NGC Chromatography Systems are also available with 10 ml system pumps .

We also offer a wide selection of columns that are compatible with all NGC Systems.

More Information

Use the module selection guide to help you decide which system is best for you.

Upgrade your NGC Chromatography System by adding more modular components to match your changing throughput and applications requirements:

• More sophisticated detection capabilities
• pH monitoring
• Column scouting
• Buffer blending

If you prefer to customize your NGC System using our online tool, configure your personalized NGC™ Instrument .