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NGC Quest 10 Plus Chromatography System by Bio-Rad
Status:
Reviewer
Member since: 2021
Organization: UMASS MEDICAL SCHOOL
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- Ease of use
- After sales service
- Value for money
Akta better watch their back.
Application Area: Protein Purification and Analysis
"When it comes to FPLC, I was always an Akta guy but this instrument changed my mind. The NGC is extremely well designed, the software is better that Unicorn (and it's free to install on other computers for analyses), and it's significantly more affordable. I wouldn't have believed it if we hadn't demoed the machine ourselves first but I 100% recommend these instruments."
Review date: 25 Jun 2021 | NGC Quest 10 Plus Chromatography System
The NGC™ Quest 10 Plus Chromatography System is designed to suit smaller labs with basic purification needs. The NGC Quest 10 Plus instrument has automated 10 ml/min pumps that provide accurate gradients for high-resolution separations for any application. The NGC Quest 10 Plus system has a multi-wavelength detector with simultaneous four-wavelength monitoring for high-accuracy detection of proteins, peptides, and nucleic acids combined with conductivity measurements. Fractionated samples can be easily collected from analytical- to preparative-scale purifications using the NGC Fraction Collector and BioFrac™ Fraction Collector . To learn more, watch the NGC system tour.
The NGC Quest 10 Plus system includes:
- 1 NGC two-tier base frame
- 2 NGC F10 pump modules for a flow rate of 0.001–10 ml/min at 3,650 psi (252 bar, 25.2 MPa)
- 1 NGC mixer module small (263 µl), includes an extension mixer barrel (750 µl)
- 1 NGC multi-wavelength detector module for simultaneous four-wavelength detection at 190–800 nm and salt gradient monitoring with integrated conductivity monitor, includes tubing and fittings and one 5 mm flow cell
- 1 NGC sample inject valve (sample inject valve kit) for automated application of small sample volumes from sample loops or large sample volumes using an optional sample pump
- 1 integrated system touch screen
- 1 copy of ChromLab™ software for instrument control, data collection, and data analysis
- 1 documentation CD containing manuals for use with NGC systems
- 2 NGC blank modules
- 1 NGC buffer tray
- 1 NGC communication adaptor for connecting the BioFrac fraction collector
Features and Benefits of the NGC Quest 10 Plus Chromatography System
A flexible, customizable chromatography system to suit both your application and workflow needs:
- The Guided Fluidics selection tool (patent pending) coupled with the Module Discovery system enables a single-click application-based system setup through automated module function recognition
- The Tier Rotate™ design allows optimal placement of valves and detectors to minimize the flow path
- Compact footprint – fits in a refrigerator
- Point-to-Plumb™ lighting shows the step-by-step LED-guided plumbing of the system
- Unique ChromLab software provides powerful system control, automated methods, and analysis options
Applications and Uses of Medium-Pressure Chromatography Systems
Preparative- to analytical-scale isolation, purification, and analysis of multiple types of molecules:
- Recombinant protein purification and refolding
- Monoclonal antibody purification
- Removal of viruses, protein preparations
- Analysis of plasma proteins for disease diagnosis
More Information
NGC modular components allow your chromatography system to meet both laboratory space and application requirements. As these requirements change, systems may be easily reconfigured and seamlessly upgraded with increased functionality, such as higher flow rates , more sophisticated detection capabilities , pH monitoring , column scouting , and buffer blending . Use the configurator tool to build the best system to fit your needs.
A PC controller enables easy communication with the workstation and peripheral devices. The controller includes the Windows 7 operating system, application software, a keyboard, mouse, and high-resolution flat-panel monitor.
