NEXTflex™ Rapid Directional qRNA-Seq™ Kit by Bioo Scientific
Manufacturer Bioo Scientific
The patent pending NEXTflex™ Rapid Directional qRNA-Seq™ Kit enables high precision directional gene expression analysis by RNA-seq.
This kit generates directional RNA-seq libraries equivalent to conventional stranded RNA-seq libraries, but with the added feature of molecular indexing. The NEXTflex Rapid Directional qRNA-Seq Kit can be used to obtain stranded information which identifies the specific DNA strands a given RNA transcript was derived from.The NEXTflex Rapid Directional qRNA-Seq Kit... Read more
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NEXTflex™ Rapid Directional qRNA-Seq™ Kit
The patent pending NEXTflex™ Rapid Directional qRNA-Seq™ Kit enables high precision directional gene expression analysis by RNA-seq.
This kit generates directional RNA-seq libraries equivalent to conventional stranded RNA-seq libraries, but with the added feature of molecular indexing. The NEXTflex Rapid Directional qRNA-Seq Kit can be used to obtain stranded information which identifies the specific DNA strands a given RNA transcript was derived from.
The NEXTflex Rapid Directional qRNA-Seq Kit is designed to prepare directional, strand-specific RNA libraries for sequencing using Illumina® sequencers while enabling the high precision measurement of RNA molecules. The NEXTflex Rapid Directional qRNA-Seq Kit efficiently generates libraries equivalent to conventional directional, strand-specific RNA-Seq libraries, but with the added feature of Molecular Indexing™, a DNA labeling technology developed by Cellular Research, Inc. This kit contains a set of 96 distinct molecular labels on the sequencing adapters. Each label consists of an 8 nucleotide barcode tag. During the ligation reaction, each cDNA fragment independently and randomly ligates to a single label from this pool of 96 adapters to result in a total of 96 x 96 = 9,216 possible combinations across both ends.
Features:
This kit generates directional RNA-seq libraries equivalent to conventional stranded RNA-seq libraries, but with the added feature of molecular indexing. The NEXTflex Rapid Directional qRNA-Seq Kit can be used to obtain stranded information which identifies the specific DNA strands a given RNA transcript was derived from.
The NEXTflex Rapid Directional qRNA-Seq Kit is designed to prepare directional, strand-specific RNA libraries for sequencing using Illumina® sequencers while enabling the high precision measurement of RNA molecules. The NEXTflex Rapid Directional qRNA-Seq Kit efficiently generates libraries equivalent to conventional directional, strand-specific RNA-Seq libraries, but with the added feature of Molecular Indexing™, a DNA labeling technology developed by Cellular Research, Inc. This kit contains a set of 96 distinct molecular labels on the sequencing adapters. Each label consists of an 8 nucleotide barcode tag. During the ligation reaction, each cDNA fragment independently and randomly ligates to a single label from this pool of 96 adapters to result in a total of 96 x 96 = 9,216 possible combinations across both ends.
Features:
- Generates stranded, molecular indexed RNA-seq libraries
- Enables high precision, quantitative measurement of RNA expression
- Enhanced Adapter Ligation Technology offers a larger number of unique sequencing reads
- Input 10 - 100 ng of mRNA or rRNA-depleted total RNA
- Embedded sample index barcodes for multiplexing of up to 96 samples
- Automation-friendly workflow is compatible with liquid handlers
- Functionally validated with Illumina sequencing platforms
Product Overview
NEXTflex™ Rapid Directional qRNA-Seq™ Kit
Manufacturer Bioo Scientific
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