NEXTflex™ DNA Modules for NGS Library Preparation by Bioo Scientific
Manufacturer Bioo Scientific
The NEXTflex™ DNA Modules offer additional flexibility to next generation sequencing library preparation. All of these modules are compatible with the Illumina Genome Analyzer IIx, HiSeq and MiSeq sequencing platforms. These modules are available in any quantity greater than 100 reactions. NEXTflex™ DNA End Repair Module The NEXTflex™ DNA End-Repair Module has been designed with enzymes and buffers to efficiently repair fragmented DNA with overhangs to blunt ended DNA. The NEXTflex™ DNA End Repa... Read more
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NEXTflex™ DNA Modules for NGS Library Preparation
Average Rating: 5.0
1 Scientist has reviewed this product
Status:
Reviewer
Member since: 2014
Organization: University of Sussex
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- Ease of use
- After sales service
- Value for money
Rating: 5.0
Application Area: Hi-Seq libraries
"Easy to use and it is good that we can buy individual components."
Review date: 20 Aug 2014 | NEXTflex™ DNA Modules for NGS Library Preparation
The NEXTflex™ DNA Modules offer additional flexibility to next generation sequencing library preparation. All of these modules are compatible with the Illumina Genome Analyzer IIx, HiSeq and MiSeq sequencing platforms. These modules are available in any quantity greater than 100 reactions.
NEXTflex™ DNA End Repair Module
The NEXTflex™ DNA End-Repair Module has been designed with enzymes and buffers to efficiently repair fragmented DNA with overhangs to blunt ended DNA. The NEXTflex™ DNA End Repair Module is supplied as a master mix to maximize efficiency and convenience in DNA sample preparation workflows. The module is the first step in the NEXTflex™ DNA Sequencing Kit.
NEXTflex™ DNA Adenylation Module
The NEXTflex™ DNA Adenylation Module has been designed with enzymes and buffers to efficiently incorporate an “A” on the 3’ end of blunt DNA fragments. The module is the second step in the NEXTflex™ DNA Sequencing Kit.
NEXTflex™ DNA Ligation Module
The NEXTflex™ DNA Ligation Module has been designed with enzymes and buffers to efficiently ligate DNA adapters to adenylated DNA fragments. Using Bioo Scientific’s “Enhanced Ligation Technology”, adapters are bound to more DNA fragments leading to increased sequencing diversity and reads. The module is the fourth step in the NEXTflex™ DNA Sequencing Kit.
NEXTflex™ DNA PCR Module
The NEXTflex™ DNA PCR Module has been designed with enzymes, buffers and nucleotides to efficiently amplify adapter ligated DNA. The module is the final step in the NEXTflex™ DNA Sequencing Kit.
NEXTflex™ DNA End Repair Module
The NEXTflex™ DNA End-Repair Module has been designed with enzymes and buffers to efficiently repair fragmented DNA with overhangs to blunt ended DNA. The NEXTflex™ DNA End Repair Module is supplied as a master mix to maximize efficiency and convenience in DNA sample preparation workflows. The module is the first step in the NEXTflex™ DNA Sequencing Kit.
NEXTflex™ DNA Adenylation Module
The NEXTflex™ DNA Adenylation Module has been designed with enzymes and buffers to efficiently incorporate an “A” on the 3’ end of blunt DNA fragments. The module is the second step in the NEXTflex™ DNA Sequencing Kit.
NEXTflex™ DNA Ligation Module
The NEXTflex™ DNA Ligation Module has been designed with enzymes and buffers to efficiently ligate DNA adapters to adenylated DNA fragments. Using Bioo Scientific’s “Enhanced Ligation Technology”, adapters are bound to more DNA fragments leading to increased sequencing diversity and reads. The module is the fourth step in the NEXTflex™ DNA Sequencing Kit.
NEXTflex™ DNA PCR Module
The NEXTflex™ DNA PCR Module has been designed with enzymes, buffers and nucleotides to efficiently amplify adapter ligated DNA. The module is the final step in the NEXTflex™ DNA Sequencing Kit.
Benefits
• Flexible options – the NEXTflex™ DNA Modules are available in aliquots containing any number of reactions you need
• Convenient – Reactions are provided in master mix format to reduce steps during DNA sample prep workflows
• Enhanced Adapter Ligation Technology offers a larger number of unique sequencing reads
• Automation-friendly workflow is compatible with liquid handlers
• Compatible with targeted sequence capture
• Functionally validated with Illumina GAIIx, HiSeq and MiSeq sequencing platforms
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