NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® by New England Biolabs Inc.

NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for downstream applications such as next generation sequencing and expression library construction. The reagents included within these master mix sets must pass rigorous quality control standards and are lot controlled, both individually and as a set of reagents.

Each set of reagents is functionally validated together through construction and sequencing of an RNA library on the Illumina sequencing platform.

NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® Features:

• Accurate retention of transcript strand of origin information
• Utilizes dUTP-based methodology
• Low input amounts (as low as 10ng purified mRNA or ribosomal-depleted RNA, or 100ng Total RNA)
• Fast workflow (4.5 – 5 hours), with minimal hands-on time
• Robust, reliable performance
• Ultra high fidelity amplification with minimized GC bias

NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® Includes:

• NEBNext First Strand Synthesis Reaction Buffer (5X)
• Random Primers
• ProtoScript® II Reverse Transcriptase (200,000 units/ml)
• Murine RNase Inhibitor
• NEBNext Second Strand Synthesis Enzyme Mix
• NEBNext Second Strand Synthesis Reaction Buffer with dUTP Mix (10X)
• NEBNext End Prep Enzyme Mix
• NEBNext End Repair Reaction Buffer (10X)
• Blunt/TA Ligase Master Mix
• Nuclease-free Water
• NEBNext High-Fidelity 2X PCR Master Mix (2X)
• NEBNext USER Enzyme