NEBNext® mRNA Library Prep Master Mix Set for Illumina® by New England Biolabs Inc.

NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for downstream applications such as next generation sequencing and expression library construction. The reagents included within these master mix sets must pass rigorous quality control standards and are lot controlled, both individually and as a set of reagents.

Each set of reagents is functionally validated together through construction and sequencing of an RNA library on the Illumina sequencing platform.

NEBNext® mRNA Library Prep Master Mix Set for Illumina® Includers:

• NEBNext RNA Fragmentation Buffer (10X)
• NEBNext RNA Fragmentation Stop Solution (10X)
• Linear Acrylamide (10 mg/ml)
• Random Primers
• Murine RNase Inhibitor
• NEBNext First Strand Synthesis Reaction Buffer (5X)
• NEBNext Second Strand Synthesis Enzyme Mix
• NEBNext Second Strand Synthesis Reaction Buffer (10X)
• NEBNext End Repair Enzyme Mix
• NEBNext End Repair Reaction Buffer (10X)
• NEBNext dA- Tailing Reaction Buffer (10X)
• Quick T4 DNA Ligase
• NEBNext Quick Ligation Reaction Buffer (5X)
• Nuclease-free Water
• Phusion High-Fidelity PCR Master Mix with HF Buffer (2X)
• NEBNext High-Fidelity 2X PCR Master Mix (2X)
• ProtoScript® II Reverse Transcriptase (200,000 units/ml)
• Klenow Fragment (3´→ 5´ exo–)
• Deoxynucleotide Solution Mix (10 mM)