Thermo Scientific™ NanoDrop™ 2000/2000c Spectrophotometers by Thermo Fisher Scientific

"We demoed several small volume spectrophotometers, and while the Molecular Devices product had superior software and user interface, the NanoDrop from Thermo Fisher by far had the best hardware and at the end of the day it is the hardware itself that matters. We tested several other off brand ones and they were so terrible they are not even worth mentioning. While I preferred the Molecular Devices software, the Thermo software wasn't terrible so it was perfectly adequate."

"Great product, helps massively with workflow."

"For routine quantification, the NanoDrop 2000 is an excellent instrument and is also very reliable. My only minor criticism is that the software could be improved to be more intuitive, particularly for recall and export/printing of data. However, for custom methods the NanoDrop comes into its own. As a protein biochemist, I often want to measure a micromolar concentration of purified protein by UV, based on the extinction coefficient of that particular protein. The NanoDrop is the ONLY instrument we have tried that allows such custom method setup. Once again, the interface for custom method setup is not the easiest or most intuitive that it could be, but it adds powerful functionality that is not presently accessible with competitors' products, to my knowledge."

"The nanodrop is just about the easiest to use piece of equipment in the lab. 1.5uL of specimen on the pedestal, press measure, boom! Done!"

"It’s very convenient and potential application for my research. The ease of operation and flexibility in the software manipulation is remarkable"

"Easy to use. Repeatable results. Easy for multiple users to track results."

"The nanodrop made quantitation of my DNA preps and Protein Purification easy, with minimal use of sample required. I found that 1.5-2ul of sample was best for achieving consistent results."

"Only microvolume spectrophotometers that measure sample volumes as small as 0.5µL."

"The instrument is very reliable and easy to use, even for undergraduates. We constantly use it in the undergraduate teaching labs and research. I don't know about the service because we have not had problem to call for service. We are writing grants to get two more. The results are consistent and accurate compared the the gel data."

"Excellent performance, incredible consistency, and sensitivity is amazing"

"This product was very easy to use. There is a great GUI and samples are easy to work. i would recommend this to all labs."

"Easy to use with reproducible results. Always the equipment of choice for DNA or RNA quantification."

"i like the design of the machine. Unlike Nanodorp 2000/c, we do not need PC so that we can save space. Starting up the machine takes a bit too long. If we can do kinetic measurements of spectra (not absorbance of a single wavelength), it would be better."

"We Engineer proteins to sense various analytes in whole blood by using fluorescence . After we engineer we need to see if they respond by looking at the fluorescence signal. We use Nanodrop2000 a lot to read the absorbance of different fluorophores we crosslink to our sensors."

"This device is very very good for reading the concentration/OD of materials such as peptides, antibodies, proteins, etc. All of these are measured down around 250-300nm. As long as you remember to run the samples fast, in this range the device works PERFECTLY! The issue is for antibody-dye conjugates where one of the peaks is above 600nm, it starts to get a bit non-linear and by the time you reach 650nm it is significantly non-linear (to the point that it threw off our calculations). As such, it still had great use in quantifying antibody conc, something we did many times a day just not for final conjugates (such as Alexa 647 or Alexa 750)."

"For me the best devise to determine concentration of DNA, RNA or protein. You need very few materials. Very easy to use and the maintenance is minimum, and it doesn't take much space in the lab!"

"Can quantify DNA, RNA and Protein in a very small volume. Easy to use."

"This is the latest Nanodrop version we are currently using in our core facility. You do not need to log into a computer for use and appears more efficient. Much more user friendly."

"Very effective for measuring pure nucleic acids above 10ng/ul. Does not perform well with contaminants in samples or dilute nucleic acid solutions below 10ng/ul. Consistent for OD600 cell culture measurements when using the microvolume pedestal. But need to multiply the reading x10 to take into account the path length."

"NanoDrop is very easy to use and only one micro-liter volume of sample is required. The concentration measurement of nucleic acids by NanoDrop is pretty accurate and highly reproducible when the concentration is more than 10 nanogram per micro-liter. As a UV-Vis Spectrometry tool, NanoDrop can also be used in other purposes. "

"We use the NanoDrop for HT concentration determination- the minimal sample volume and HT nature make it extremely well suited for our needs. Being data driven, the rapid turnaround time makes this system critical for our day to day needs. Thermo was extremely helpful during and after the sales process. Having used this system previously, the decision to acquire it was simple. They continue to provide excellent after care service and support as well."

"Thermo was extremely helpful during and after the sales process. Having used this system previously, the decision to acquire it was simple. They continue to provide excellent after care service and support as well."

"Nanodrop needs tiny amounts of sample, helped us investigating product based process impurity profiling, helped us in process development consuming less sample giving important and greater results of consistency. We greatly appreciate the design and dependability of the system"

"This product is easy to use and the data is really accurate. "

"It is very easy to use and analyse my sample with small volumes in a short time. "

"Very sensitive and easy to use. Reproducible results. Small amount of samples needed. Nobody would return to cuvettes spectrophotometer after testing Nanodrop"

"It is a very good instrument, stress free when it comes to measuring DNA/RNA, but unfortunately we have not have much luck with the maintenance."

"With the NanoDrop 1000 it has become simple to quantify small doses of low concentration RNA . Avoiding waste of material"

"This product makes it simple to determine the protein concentration of a tumor cell suspension and allows us to process, aliquot and freeze in a timely and efficient manner."

"Easy to use, simple and straightforward, as it ages, there could be various intermittent issues though."

"This product is handy and very easy to use. It offers quick and accurate readings. Out of the spectrometers that I used in the past, this is the most reliable machine I have ever used. Somebody can easily measure DNA, RNA of different forms and cell cultures without making a major physical change."

"This is a valuable device with user friend software, very simple to work and accurate results and reproducible data."

"It is not complicated and can achieve very fast results. There is no need for additional equipment such as cuvettes. Software is intuitively designed and is ideal for small quantities, such as nano drops. I have had the machine for three years and I have had no problems with it. I have only ever had positive experiences with the distributor too (peqlab)."

"Nanodrop has become a common lab item for extensive use in measuring concentration of biomolecules. The ease of use, no need for cuvettes and need of low volumes was unprecedented and was the reason of nanodrop success. However, the issues of reproducibility and reliability remain a big concern. "

"This nano-drop has 8 pedestals which is great for running multiple samples. After having this instrument for a few years I saw that it wasn't producing consistent results. I would load the same samples multiple times (making sure they were homogenous and freshly mixed) and I would get substantial differences in results. I called the support line and the people were very friendly, helpful and followed up. They sent us a reconditioning kit, which we applied immediately and it seemed to take care of the problem. Make sure you always run your samples in duplicate to make sure your machine is consistent. The machine and it's accompanying software are pretty easy to use. We like this device a lot and would recommend it. You do have to make sure you have good technique and that you are able to load all 8 samples fast enough before any of your samples evaporate- causing a failure. With this device I highly recommend reading the instruction manual."

"The "Nanodrop nd 1000" and the Software is very easy to handle. The documentation of the results is pretty easy. But I think the nanodrop has one weak point, the range (raw 340 sometimes has a too big measurement of the range). However it is a nice product."

"Very useful device with small sample volumes. The device is a number of years old and still working well. The pedestal is easy to clean, and the R/DNA values are very repeatable. The protein results are variable, but that is to be expected."

"Very easy to use, and does not take much instruction prior to first use. Fast analysis and simple software, with easy-to-understand readout. I would recommend this to a colleague."

"Easy to use, with clear directions. Easy to clean and little to no problems related to malfunctions with computer technology. When using this instrument for determining the concentration of RNA or DNA in a sample, the directions are straight forward and the numbers are clearly presented. I would recommend this product to future colleagues as well as other students that need to use it."

"It is an excellent product. The services of this equipment are excellent. The quality is very good and all of our clients are very comfortable with this equipment."

"This spectrophotometer is very easy to use for measuring DNA and RNA concentration. You only need take 1µl from your samples and you can know the concentration within seconds. It's also very easy to clean."

"This instrument is very interesting for nanotechnology, mainly for use the characterization of gold and silver nanoparticles in solution. I have been looking to understand your performance and calculate figures of merit such as selectivity, sensibility, limit of detection and quantification, robust, and others."

"This product is really good, especially small and very convenient to use.  We are using this product for measuring PCR products after PCR or DNA or RNA analysis after ethanol precipitation and gel extraction.  The quality of data is very good and excellent.  It's really good for requiring small amount of samples."

"This product was a little pricey but is easy to use even for undergraduates. It is a solid performer and works well every time. We have been pleased with its stability. All in all, worth the extra money."

"The NanoDrop 2000 is an excellent, easy-to-use instrument that makes DNA/Protein quantification a pleasure.  We are a teaching institution, so the NanoDrop is a perfect tool for allowing our students to quantitate and evaluate the quality of the DNA and proteins that they purify.  The most attractive features of the NanoDrop are the small volumes of sample required for analysis (as little as 1 microL) and the fact that no cuvettes are required.  The instrument itself is easy to use and the software that accompanies it for control and data analysis is very user friendly.  This is one of the best pieces of equipment that our lab has purchased!"

"It was easy to use, user friendly, and saves lots of time. In the field of neurotransmitters and RNA/DNA where small amounts of samples are used the Nanodrop spectrophotometer is really useful."

"This spectrophotometer is miniature, easy to use, saves samples and time. Excellent at saving space in the lab, and most of all affordable."

"On the plus side, this spectrophotometer is very simple to use, can measure as little as 10 ng/microl of DNA (the manufacturer advertise 2 ng/microl, but in my hands the measurements are not to be trusted at concentrations below 10 ng/microl), and does not require prior dilution of the sample (1 microl is needed for the measurement). On the minus side, the sample needs to be of high purity in aqueous solution devoid of surfactant (for example no SDS). In this regard, I have found this spectrophotometer not well suited for protein concentration measurements."

"Low volume, easy to use. Reliable, with quick readings and accurate."

"Very quick and simple to use, it gives reproducible results. I would reccomend this equipment to a colleague."

"The single channel nanodrop is quick and easy to use, informing you of the concentration of your sample within just a few seconds, therefore it's possible to determine concentrations of many different samples quickly. When repeating same samples, concentration is usually extremely accurate and therefore a trustworthy and reliable machine."

"Small sample required (2ul), quick and robust."

"I like how easy it is to use but I would like the 'data export' function to be more visible, otherwise it works very well."

"Compact and easy to use. Only requires a small amount of sample. However the price is high and it can only run one sample at a time. Also in order to get repairs or service it needs to be shipped back to manufacturers."

"Very easy to operate, even for first time user. Only a small volume of sample is required. The software-user interface is straight forward and easy to follow."

"A very user-friendly interface with good details and walk-throughs for various protocols and applications. Easy to get up and running in a few minutes. Data is displayed in a very clear format. Easy to customize and save my own protocols, data, etc. I can tweak methods very precisely and easily. Changes are easy to detect. I have some difficulty with reproducibility - some sample measurements have to be thrown out for no apparent reason. The instrument has to be recalibrated frequently."

"Very small sample size, easy calibration and wide dynamic range. No need to dilute samples, can do oligonucleotides. Protein, double stranded DNA, wide range of applications."

"Very easy to use and fast sample time. Program on computer is very good and easy to use. Only need to use minimum sample volume which is very convenient. It's small, does not take much space and is very easy to manage/clean."

"Easy to use and quantitative. Limitations: It can't be read when there is an air bubble in it and there is no temperature control. It can only do samples, one by one, and there is no array detection."

"This is easy to use and the device is a great addition to any lab. The small sample size makes this device applicable to many of our small volume microfluidic samples."

"This equipment is easy to use and does not require training. We purchased it four years ago and it is still working fine. The calibration requires the purchase of a calibration fluid but is easy to do."

"Nanodrop provides a quick and accurate method to assay protein, DNA and cell counting. Very easy interface. Instrument is small and connects via desktop and laptop."

"An absolute joy to use, it hasn't failed yet, however it doesn't replace a standard UV-VIS spec. You 'could' take the same measurements with a conventional spec- which gives better measurements- but requires a larger sample size."

"Great machine for DNA/RNA labs. Easy measurement without dilution. Simple to use."

"Great for low volume. gives accurate absorbance tests."

"This nanodrop is very easy to use and the data are compatible with Biorad nanodrop."

"Very easy to use with reproducible results."

"Low sample volume with excellent consistency."

"Easy quantification of protein and DNA concentration. Also easy for looking at the ratio of dye to protein."

"simple to use. efficient and has very few problems if cleaned as specified by the manual."

"Small size and easy to use."

"Easy to use and great across many applications. Be sure to calibrate whenever results become untrustworthy as it will occasionally fall out of line, but calibration will bring it right back in."

"measurements vary even with the same samples."

"Simple to use and provides accurate info."

"A very good spectrophotometer."

"This is a great spectrophotometer, especially for quantifying small amounts of RNA or DNA."