Monolith NT.115 Instruments by NanoTemper Technologies

"This instrument is by far the most sensitive piece of equipment to measure the interaction between small molecule and protein or DNA. It is super easy to use and needs a minimum amount of protein. The control software contains very detailed instruction, and need new users need no training. The instrument is completely maintenance-free. Strongly recommend!"

"For us it is very important that we are able to measure the affinity of protein interactions from small amount of recombinant proteins, which is a big advantage of the Monolith instrument. The fast, cca 15 min assay time for one titration is a great feature too. We are pretty much satisfied with the company's customer care system, which includes free technical assistance, some on-site demos after purchase, and well-organized user meetings. The company provides help (technical materials and test measurements) to ensure good assay conditions for particular problems. The prices for consumables are acceptable. We also characterize protein interactions via surface plasmon resonance or quartz crystal microbalance, and with the Monolith instrument we can complement those measurements under similar, or even the same, conditions (concentration, pH, buffer composition, etc.). Altogether, the NT.117 is a great instrument, which does not compromise value for price."

"Using MST we obtained very convincing data for affinities of small molecules/peptides that were highly reproducible. Experimental set-up is very easy and time-efficient in contrast to some alternative methods. But not only that the technology works reliable, the instruments look extremely aesthetic. Last, but not least, the customer support is engaged and efficient."

"Our company 2bind uses this technology to study molecular interactions in terms of basic binding parameters. One major focus is on drug-target interactions. The MST offers information on binding affinity, stoichiometry and thermodynamics. The method works in solution with freedom of buffers (even measurements in sera, lysates are possible). It is rapid and consumes very low sample material due to the clever capillary format. We highly appreciate the integrated quality controls showing 1. unspecific adsorption of molecules to the glass surfaces and 2. aggregation effects of the samples. This essential information helps us to optimize the technical setup and obtain optimal data. The output data are highly comparable to ITC and have a good overlap to surface based methods such as SPR. Overall we love this technology, because the size of the interaction partners does not matter. Hence it is the perfect tool to study drug binding to a target protein. The device itself is robust, only takes very little space and the consumables are affordable. The technical support is very good. Thanks guys for your constant support. We can highly recommend this technology."

"We use Monolith NT.115 every day for protein and small molecule interaction studies. We developed a single point screen method using this machine, which helps us to screen 100's of compounds every day. Setting up experiments using this machine is very easy and it does not require elaborate training. Consumables are cheap and working cost of the machine is also very low. MO Affinity analysis software is user friendly and using the software is easy and quick."

"This is a low- to medium-throughput instrument to measure binding constants between two proteins or a protein and a small molecule ligand. The vendor offer two versions: label-based and label-free. Both instrument versions works really well and have consistently produced Kd values close to previously-reported ones or values that end up getting corroborated in the future through traditional calorimetry or SPR techniques. Sample consumption is low and the instrument is very stable (no software crashes or other type breakdown) and easy to use. In our lab, we have used the thermophoresis instruments for over a dozen distinct projects, with results published in almost 10 papers already."