MiSeq Personal Sequencer by Illumina

Manufacturer Illumina  |  Available Worldwide
4.6
/
5.0
  |  27 reviews
Illumina's MiSeq is the only fully integrated personal sequencer, delivering a streamlined solution that takes you from rapid sample prep through automated data analysis and storage in the BaseSpace cloud. Sequencing doesn’t get any easier than this. As the only personal sequencer capable of producing paired-end reads, Illumina's MiSeq system puts the largest portfolio of sequencing applications at your fingertips. Optimized sample preparation kits, push-button sequencing, and automated data an... Read more
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MiSeq Personal Sequencer by Illumina product image
MiSeq Personal Sequencer

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Average Rating: 4.6
27 Scientists have reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2023

  • Organization: ÖGH Hanusch Hospital



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Easy to handle, great results!
Rating: 4.3

  • Application Area: Medcal genetics

"Device is super easy to handle. Helpful technical support. Most protocols for library preperation are aligned to Illumina devices like MiniSeq."

Review date: 20 Mar 2023 | MiSeq Personal Sequencer
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Eurofins Lancaster Laboratories



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great product, excellent company
Rating: 5.0

  • Application Area:NGS of amplification products of genes of interest

"Illumina products in general are exceptionally user friendly and intuitive to use. Additionally, Illumina’s customer support is superb and their help troubleshooting is extensive."

Review date: 28 Sep 2022 | MiSeq Personal Sequencer
  • Status:

    Reviewer

  • Member since: 2022

  • Organization: University for Applied Sciences Coburg



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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As always, we were very content with the service and analysis provided.
Rating: 4.7

  • Application Area:Analyzing microbial composition of car interiors

"The sequencing and analysis of microbial composition helped us to get an overview of the microbiome, which is present in car interiors. Sadly I can not say much more about results as they ought to be held in nondisclosure. Our university has a pipeline to further process data given from the analysis of microbial communities so it was very easy to use for us. The customer service also reacted fast to our request to send an estimate of cost and for further consultation."

Review date: 27 Jun 2022 | MiSeq Personal Sequencer
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: CIBION



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Best quality results on sequencing
Rating: 5.0

  • Application Area:Microbiology

"Excellent equipment with an outstanding performance in 16S sequencing."

Review date: 01 Oct 2021 | MiSeq Personal Sequencer
  • Status:

    Advanced Reviewer

  • Member since: 2020

  • Organization: Precisionantibody



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Great tool, once familiarized. It is easy to use and very useful.
Rating: 4.3

  • Application Area:Sequencing

"It is a great high-throughput technology, you can sequence multiple samples at once. We mostly use it for sequencing BCR (B cell receptor) CDR regions using kits. It is easy to use, but the sequence analysis requires some expertise from the user on the software usage and retrieving the data. The basic training given by Illumina is not sufficient enough, you may need additional training and the purchase of the software not included with the instrument."

Review date: 27 Jul 2021 | MiSeq Personal Sequencer
  • Status:

    Expert Reviewer

  • Member since: 2016

  • Organization: University of North Carolina at Charlotte



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Excellent results providing personal sequencer.
Rating: 4.3

  • Application Area:A high valuable sequencing system

"What a instrument it is. As the name indicates it is a personal sequencer with great work capacity and easy to use features. Highly recommended and this machine provides very efficient results. Good one to have in the sequencing labs."

Review date: 09 Jun 2021 | MiSeq Personal Sequencer
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: University of British Columbia



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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The results were reproducible when run by anyone in the lab!
Rating: 5.0

  • Application Area:Targeted genomic sequencing to study clonal dynamics in cancers

"We were able to very quickly set up and run a targeted sequencing pipeline to study the clonal dynamics of many human tumours and their models (patient-derived xenografts) in vivo."

Review date: 14 May 2021 | MiSeq Personal Sequencer
  • Status:

    Advanced Reviewer

  • Member since: 2020

  • Organization: Precisionantibody



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great tool to consider purchasing.
Rating: 5.0

  • Application Area:Sequencing

"Illumina Miseq is a great tool for sequencing genes or DNA of interest. The only disadvantage is it's very expensive. All the consumables and reagents are very expensive too. You should consider purchasing it only if needed and can be used to the fullest potential. Initially, it will take time to learn, but once you get used to it, it is not that hard. Tech support is great. You need a lot of support from 3rd party as well for the kits and sequence analysis software."

Review date: 07 May 2021 | MiSeq Personal Sequencer
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: Isogenica



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Fantastic instrument, great customer support!
Rating: 5.0

  • Application Area:Sequencing of DNA

"A great piece of equipment, small footprint, reliable, easy to use and provides excellent quality data (generally looking for millions of reads over a 50-300 bp range depending upon the kit used). After sales support is excellent - prompt to answer queries and in troubleshooting any issues we have had. Have been using this for several years and few if any issues that were not quickly resolved all the while generating fantastic quality data - would highly recommend to anyone looking for a smaller DNA sequencer!"

Review date: 30 Oct 2020 | MiSeq Personal Sequencer
  • Status:

    Reviewer

  • Member since: 2018

  • Organization: NARC



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Great tool.
Rating: 4.0

  • Application Area:Sequencing

"Reproducible results."

Review date: 28 Jul 2020 | MiSeq Personal Sequencer


  • Status:

    Reviewer

  • Member since: 2019

  • Organization: University of North Dakota



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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This instrument is vital to our genomics core lab.
Rating: 4.3

  • Application Area:Genomics sequencing

"This sequencer works really well. We have had a few hiccups but when there has been issues Illumina is amazing at helping either on the phone or sending personnel out to take a look at it. They have great webinars to help understand what is going on with the instrument."

Review date: 24 Jan 2020 | MiSeq Personal Sequencer
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Ualg



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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It's great.
Rating: 4.0

  • Application Area:Metagenomic analysis

"It was easy to use but we usually had problems with output quality sequences."

Review date: 22 Jul 2019 | MiSeq Personal Sequencer
  • Status:

    Reviewer

  • Member since: 2014

  • Organization: European PompidouHospital



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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We would still be on the bench without it
Rating: 4.0

  • Application Area:Sequencing

"Illumina made it easier to prepare libraries, to have a clear vision of the assay quality and in the end focus on the sequences meaning."

Review date: 11 Jun 2019 | MiSeq Personal Sequencer
  • Status:

    Reviewer

  • Member since: 2008

  • Organization: Institute of Biomedical and Genetic Engineering



  • Ease of use
    4 out of 5
    After sales service
    3 out of 5
    Value for money
    3 out of 5
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The instrument is quite-useful and there is always a margin for improvement.
Rating: 3.3

  • Application Area:Molecular research, diagnostics and forensics

"The next generation platform, Illumina MiSeq is quite useful platform and is being used very effectively in my lab in human molecular research to find the etiology of disease, exome sequencing and discovery of novel SNPs to find the etiology of various inherited disorders and also being applied in diagnostic and human DNA forensics."

Review date: 14 Mar 2019 | MiSeq Personal Sequencer
  • Status:

    Advanced Reviewer

  • Member since: 2018

  • Organization: assistance publique hôpitaux de paris



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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You just can't do without it.
Rating: 4.3

  • Application Area:Resequencing, rna seq

"It used to be very demanding to sequence Library, low the miseq system enables a piece of cake protocol. You focus on the results, not on the benchtop work"

Review date: 20 Dec 2018 | MiSeq Personal Sequencer
  • Status:

    Reviewer

  • Member since: 2018

  • Organization: Orbit Discovery



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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Good investment
Rating: 4.7

  • Application Area:NExt Generation Sequencing

"We tried the Oxford Nanopore and the one from Thermo Fisher, we came to a conclusion that the Illumina system gives more accurate read than the other two for amplicons samples. The support service is really good in terms of speed of response to requests."

Review date: 10 Oct 2018 | MiSeq Personal Sequencer
  • Status:

    Reviewer

  • Member since: 2018

  • Organization: University of westminster



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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Miseq is what I seek
Rating: 4.7

  • Application Area:Whole genome sequencing

"Very easy to use. I get fantastic support from illumina team."

Review date: 09 Oct 2018 | MiSeq Personal Sequencer
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: TGen



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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We've always gotten the results we expected. Technical support provides great service
Rating: 5.0

  • Application Area:Next Generation Sequencing, DNA, RNA, Single Cell 5',3', and T-cell and B-cell receptor assays, and

"The MiSeq is typically used to conduct test runs on certain assays before we move them unto bigger platforms like the HiSeq and 3000/4000 Instruments. Technical support has always been helpful in providing guidance whenever we run into any sample sheet issues among many others. I recently started using it to sequence my WGS cfDNA samples at low coverage and based on tumor content moving on the construction of Exome libraries. It is definitely an instrument that we cannot do without especially due to the cost."

Review date: 26 Jul 2018 | MiSeq Personal Sequencer
  • Status:

    Reviewer

  • Member since: 2014

  • Organization: IEH



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Good technical support
Rating: 4.0

  • Application Area:Genome sequencing

"Miseq is a good sequencer for microbiologist. Many of application for identifying microorganisms and easy to use machine. But longer read like 300bp has a quality issue on the read2. Other than this, everything is working as expected. Materials cost is a bit higher."

Review date: 15 Nov 2017 | MiSeq Personal Sequencer
  • Status:

    Reviewer

  • Member since: 2014

  • Organization: CDC



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great and versatile instrument!
Rating: 5.0

  • Application Area:Short read whole genome sequencing

"The MiSeq is great for short read technology. We use our short reads to "polish" our long-read PacBio assemblies due to its high coverage and we love it."

Review date: 17 Jan 2017 | MiSeq Personal Sequencer
  • Status:

    Advanced Reviewer

  • Member since: 2016

  • Organization: University of Padova



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great results with faster throughput, lower cost, and a variety of applications
Rating: 4.7

  • Application Area:Next generation sequencing

"MiSeq allows small genome sequencing and assembly, and enables detection of target variants with unmatched accuracy, especially within homopolymer regions. Now, even more samples can be processed in less time while generating more reads per run than any previous versions. All of this can be achieved using the targeted gene and small genome sequencer with the shortest sample-to-data workflow. The MiSeq instrument fills a niche for smaller genome projects with a lower cost per run, faster throughput, and a variety of applications"

Review date: 23 Dec 2016 | MiSeq Personal Sequencer
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Ovation Fertility LLC



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Very good
Rating: 4.7

  • Application Area:NGS sequencing

"Achieved excellent quality and reproducible results. Very happy with it."

Review date: 23 Jun 2016 | MiSeq Personal Sequencer
  • Status:

    Advanced Reviewer

  • Member since: 2013

  • Organization: Beth Israel Deaconess Medical Center



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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Great results and so easy to use!
Rating: 4.7

  • Application Area:Analyze small RNA

"It was easy to use and they were so helpful in data analysis"

Review date: 23 Jun 2016 | MiSeq Personal Sequencer
  • Status:

    Advanced Reviewer

  • Member since: 2013

  • Organization: Beth Israel Deaconess Medical Center



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Love the ease of use!! Thanks Illumina!!
Rating: 5.0

  • Application Area:NGS for exomes and targeted panels

"I love Illumina and the customer service they give if there is any issue with the sequencer or with the RTA software "

Review date: 24 Feb 2016 | MiSeq Personal Sequencer
  • Status:

    Advanced Reviewer

  • Member since: 2013

  • Organization: Beth Israel Deaconess Medical Center



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great Results!
Rating: 5.0

  • Application Area:NGS analysis

"It is so easy to use and great results! one can run the sample and get results in a fast and efficient manner"

Review date: 15 Feb 2016 | MiSeq Personal Sequencer
  • Status:

    Advanced Reviewer

  • Member since: 2013

  • Organization: Beth Israel Deaconess Medical Center



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Rating: 5.0

  • Application Area:NGS

"It is really good for speeding up the NGS process and has good downstream analysis options."

Review date: 06 Oct 2015 | MiSeq Personal Sequencer
  • Status:

    Reviewer

  • Member since: 2013

  • Organization: ICRISAT



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Rating: 4.3

  • Application Area:Bacterial PathoGenomics

"My personal best benchtop sequencer in terms of accuracy and efficiency. Best suitable for Bacterial Genomics as multiplexing is easier and cost effective."

Review date: 02 Jan 2014 | MiSeq Personal Sequencer
Illumina's MiSeq is the only fully integrated personal sequencer, delivering a streamlined solution that takes you from rapid sample prep through automated data analysis and storage in the BaseSpace cloud. Sequencing doesn’t get any easier than this.

As the only personal sequencer capable of producing paired-end reads, Illumina's MiSeq system puts the largest portfolio of sequencing applications at your fingertips. Optimized sample preparation kits, push-button sequencing, and automated data analysis, create the first truly end-to-end sequencing solution.

MiSeq is the only benchtop sequencer that can produce 2 x 250 paired-end reads and up to 8.5 Gb of data in a single run. Allowing you to assemble small genomes or target variants with unmatched accuracy, especially within homopolymer regions. Process more samples in less time. Generate higher read depths to get greater precision for variant detection. All using the shortest sample-to-data workflow among all benchtop sequencers.


MiSeq Personal Sequencer Features:

  • Access the world's most widely-published and accurate sequencing chemistry, backed by >4,800 peer-reviewed publications
  • Multiplex up to 384 samples per run for greater efficiency
  • Get accurate bi-directional amplicon sequencing
  • Generate more complete de novo assemblies

For In Vitro Diagnostic Use. Contact an Illumina representative for regional availability.

Product Overview

MiSeq Personal Sequencer by Illumina product image

MiSeq Personal Sequencer

Manufacturer Illumina  |  Available Worldwide

4.6 / 5.0 | 27 reviews