Agilent™s miRNA Complete Labeling and Hybridization Kit contains a novel Cyanine 3-nucleotide designed for consistently reliable results. The Cyanine 3-Cytidine biphosphate (pCp) reagent, in concert with Agilent™s miRNA microarray probe design, selectively labels and hybridizes mature miRNAs for optimal microarray performance and enhanced data confidence.
100 ng total RNA input per labeling reaction
Sufficient reagents to label and hybridize 24 microarrays (3, 8-plex miRNA microarray slides are contained within one kit)
Hybridization time is 20 hours
Contents include all enzymes and buffers, including Cyanine 3-pCp for labeling and Hi-RPM buffer necessary to perform assay
Hybridization kits should be stored at room temperature