Mini-PROTEAN® TGX Stain-Free™ Precast Gels

by Bio-Rad

4.3/5.0 (9 reviews)

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Mini-PROTEAN® TGX Stain-Free™ Precast Gels
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Mini-PROTEAN® TGX Stain-Free™ Precast Gels are based on the long-shelf-life TGX (Tris-Glycine eXtended) formulation and include unique trihalo compounds that allow rapid fluorescent detection of proteins with Bio-Rad stain-free imaging systems.  Mini-PROTEAN TGX Stain-Free Precast Gels do not contain SDS. For standard denaturing electrophoresis use sample and running buffers containing SDS. To retain native protein conformation and activity these gels can be run with sample and running buffer that do not contain SDS. Mini-PROTEAN TGX Stain-Free Gels retain Laemmli-like separation characteristics using standard sample and Tris/glycine running buffers. Protein migration patterns under non-denaturing conditions do not provide accurate molecular weight estimatio...Read more

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Reviews

9 Scientists have reviewed this product


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Average Rating: 4.3

Ease of use
5 out of 5
After sales service
4 out of 5
Value for money
4 out of 5
Rating: 4.7
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
4 out of 5
  • Review date: 27 Apr 2015

  • Application Area: Western Blot analysis

  • Mini-PROTEAN® TGX Stain-Free™ Precast Gels

"Stain Free Precast Gels are easy to use, save our time and more: 100% homogeneous gels "

Rating: 4.3
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money
4 out of 5
  • Review date:29 Nov 2013

  • Application Area:Western Blotting

  • Mini-PROTEAN® TGX Stain-Free™ Precast Gels

"This product is simple to use with great resolution. Has a nice separation for high weight proteins while still resolving the lower weight proteins as well, which is necessary for my experiments. It was a little expensive, but necessary for my experiments so worth it. "
Rating: 4.7
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
4 out of 5
  • Review date:25 Jul 2011

  • Mini-PROTEAN® TGX Stain-Free™ Precast Gels

"Great customer service. Easy to use and store. The gels are great, there is a good variety and they are easy to unlock. Great for Westerns."
Rating: 3.0
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money
1 out of 5
  • Review date:29 Jun 2011

  • Mini-PROTEAN® TGX Stain-Free™ Precast Gels

"Although now with increased lifespan ( 1yr versus 9 weeks before) the precast gels don't offer much value for money compared to the handmade ones."

Description

Mini-PROTEAN® TGX Stain-Free™ Precast Gels are based on the long-shelf-life TGX (Tris-Glycine eXtended) formulation and include unique trihalo compounds that allow rapid fluorescent detection of proteins with Bio-Rad stain-free imaging systems. 

Mini-PROTEAN TGX Stain-Free Precast Gels do not contain SDS. For standard denaturing electrophoresis use sample and running buffers containing SDS. To retain native protein conformation and activity these gels can be run with sample and running buffer that do not contain SDS.

Mini-PROTEAN TGX Stain-Free Gels retain Laemmli-like separation characteristics using standard sample and Tris/glycine running buffers. Protein migration patterns under non-denaturing conditions do not provide accurate molecular weight estimations.

The trihalo compounds in Mini-PROTEAN TGX Stain-Free Gels react with tryptophan residues in proteins in a UV-induced 1-minute reaction to produce fluorescence. The fluorophores remain covalently bound to the proteins throughout electrophoresis and blotting and may be subsequently visualized in gels or on PVDF membranes multiple times for validation in a western blotting workflow. The use of stain-free imaging also allows for the normalization of bands to the total protein on a blot, eliminating the use of housekeeping proteins or stripping and reprobing.

 

Features & Benefits:

  • Run times as short as 15 minutes
  • Transfer proteins in as little as 15 minutes; verify transfer to membrane with a stain-free enabled imager
  • Inexpensive Laemmli buffer system, low running costs
  • Gel images and complete analysis in less than 5 minutes after electrophoresis
  • Comparable sensitivity to Coomassie stain
  • Better reproducibility and quantitation compared to staining procedures

Applications and Uses of Mini-PROTEAN TGX Stain-Free Precast Gels:

  • 1-D polyacrylamide gel electrophoresis for protein separation
  • Accurate molecular weight estimation
  • Robust system for complex samples
  • One gel for western blotting, staining, and mass spectrometry

Product Overview

Product Image

Mini-PROTEAN® TGX Stain-Free™ Precast Gels

by Bio-Rad | Available Worldwide

4.3/5.0 (9 reviews)
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money
4 out of 5






Receive your quote directly from Bio-Rad for Mini-PROTEAN® TGX Stain-Free™ Precast Gels