Status:
Advanced ReviewerMember since: 2013
Organization: University of Penn
These are best for high molecular weight proteins.
Application Area: Run and analyze proteins on the gel
"One of the best quality gels in the market right now. "
Status:
Expert ReviewerMember since: 2010
Organization: Uni of Miss
Great results and easy to use
Application Area:Protein samples for western blotting and protein analysis.
"TGX stain free precast gels are easy use and handle. Analyze proteins in sample without staining before and after western blotting. "
Status:
ReviewerMember since: 2014
Organization: University of P.J. Šafarik, Faculty of Science, UBEV, Moyzesova 11, 041 54 Košice, Slovakia
Application Area:Western Blot analysis
"Stain Free Precast Gels are easy to use, save our time and more: 100% homogeneous gels "
Status:
ReviewerMember since: 2013
Organization: Brain Research Center- The University of British Columbia
Application Area:Western Blotting
"This product is simple to use with great resolution. Has a nice separation for high weight proteins while still resolving the lower weight proteins as well, which is necessary for my experiments. It was a little expensive, but necessary for my experiments so worth it. "
Status:
ReviewerMember since: 2011
Organization: KANSAS STATE UNIV.
Application Area:Cell Biology
"Very good consistency, easy to assemble. However, in my opinion, it is a bit too expensive."
Status:
ReviewerMember since: 2011
Organization: KANSAS STATE UNIVERSITY
"Great customer service. Easy to use and store. The gels are great, there is a good variety and they are easy to unlock. Great for Westerns."
Status:
ReviewerMember since: 2011
Organization: ST BARTHOLOMEWS HOSPITAL
"Although now with increased lifespan ( 1yr versus 9 weeks before) the precast gels don't offer much value for money compared to the handmade ones."
Mini-PROTEAN® TGX Stain-Free™ Precast Gels are based on the long-shelf-life TGX (Tris-Glycine eXtended) formulation and include unique trihalo compounds that allow rapid fluorescent detection of proteins with Bio-Rad stain-free imaging systems.
Mini-PROTEAN TGX Stain-Free Precast Gels do not contain SDS. For standard denaturing electrophoresis use sample and running buffers containing SDS. To retain native protein conformation and activity these gels can be run with sample and running buffer that do not contain SDS.
Mini-PROTEAN TGX Stain-Free Gels retain Laemmli-like separation characteristics using standard sample and Tris/glycine running buffers. Protein migration patterns under non-denaturing conditions do not provide accurate molecular weight estimations.
The trihalo compounds in Mini-PROTEAN TGX Stain-Free Gels react with tryptophan residues in proteins in a UV-induced 1-minute reaction to produce fluorescence. The fluorophores remain covalently bound to the proteins throughout electrophoresis and blotting and may be subsequently visualized in gels or on PVDF membranes multiple times for validation in a western blotting workflow. The use of stain-free imaging also allows for the normalization of bands to the total protein on a blot, eliminating the use of housekeeping proteins or stripping and reprobing.
Features & Benefits:
Applications and Uses of Mini-PROTEAN TGX Stain-Free Precast Gels:
Manufacturer Bio-Rad | Available Worldwide
4.3 / 5.0 | 11 reviews