Mini-PROTEAN® TGX Stain-Free™ Precast Gels by Bio-Rad
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Status:
Member since: 2013
Organization: University of Penn
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- Ease of use
- After sales service
- Value for money
These are best for high molecular weight proteins.
Application Area: Run and analyze proteins on the gel
"One of the best quality gels in the market right now. "
Review date: 03 Jun 2016 | Mini-PROTEAN® TGX Stain-Free™ Precast Gels
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Reviewer
Member since: 2013
Organization: University of Mississippi
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- Ease of use
- After sales service
- Value for money
great results
Application Area:Protein expression analysis
"Easy to handle and save mere time on running gels. "
Review date: 20 May 2016 | 10% Mini-PROTEAN® TGX Stain-Free™ Gel, 15 well, 15 µl (456-8036)
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Advanced Reviewer Member since: 2010
Organization: Uni of Miss
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- Ease of use
- After sales service
- Value for money
Great results and easy to use
Application Area:Protein samples for western blotting and protein analysis.
"TGX stain free precast gels are easy use and handle. Analyze proteins in sample without staining before and after western blotting. "
Review date: 16 Feb 2016 | Mini-PROTEAN® TGX Stain-Free™ Precast Gels
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Reviewer Member since: 2014
Organization: University of P.J. Šafarik, Faculty of Science, UBEV, Moyzesova 11, 041 54 Košice, Slovakia
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- Ease of use
- After sales service
- Value for money
Application Area:Western Blot analysis
"Stain Free Precast Gels are easy to use, save our time and more: 100% homogeneous gels "
Review date: 27 Apr 2015 | Mini-PROTEAN® TGX Stain-Free™ Precast Gels
Status:
Advanced Reviewer Member since: 2014
Organization: Zydus Cadila VTC
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- Ease of use
- After sales service
- Value for money
"1.No trouble of hand casting. So no confusion in chemical addition. 2.Time saving. 3.Good results when compared with conventional staining. "
Review date: 21 Nov 2014 | 10% Mini-PROTEAN® TGX Stain-Free™ Gel, 15 well, 15 µl (456-8036)
Status:
Reviewer Member since: 2005
Organization: North Eastern University
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- Ease of use
- After sales service
- Value for money
Application Area:Protein Analysis In PAGE And Western Blot
"This gel has a long shelf-life, is easy to use, provides a great separation in a short time and relative not very expensive. "
Review date: 07 Nov 2014 | 10% Mini-PROTEAN® TGX Stain-Free™ Gel, 10 well, 30 µl (456-8033)
Status:
Reviewer Member since: 2014
Organization: DOC/Bureau of Industry & Security
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- Ease of use
- After sales service
- Value for money
Application Area:Protein Chemistry
"The pre-made gel is very easy to use. It has made electrophoresis extremely easy and convenient without spending hours preparing gels for an experiment. In addition, it provides consistency between experiments done at different times. "
Review date: 21 Mar 2014 | 4–20% Mini-PROTEAN® TGX Stain-Free™ Gel, 15 well, 15 µl (456-8096)
Status:
Reviewer Member since: 2013
Organization: Brain Research Center- The University of British Columbia
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- Ease of use
- After sales service
- Value for money
Application Area:Western Blotting
"This product is simple to use with great resolution. Has a nice separation for high weight proteins while still resolving the lower weight proteins as well, which is necessary for my experiments. It was a little expensive, but necessary for my experiments so worth it. "
Review date: 29 Nov 2013 | Mini-PROTEAN® TGX Stain-Free™ Precast Gels
Status:
Reviewer Member since: 2011
Organization: KANSAS STATE UNIV.
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- Ease of use
- After sales service
- Value for money
Application Area:Cell Biology
"Very good consistency, easy to assemble. However, in my opinion, it is a bit too expensive."
Review date: 19 Jan 2012 | Mini-PROTEAN® TGX Stain-Free™ Precast Gels
Status:
Reviewer Member since: 2011
Organization: Montreal Neurological Institut
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- Ease of use
- After sales service
- Value for money
"Using these gels saves a lot of time, the Westerns are very specific."
Review date: 19 Dec 2011 | Mini-PROTEAN® TGX Stain-Free™ Precast Gels
Status:
Reviewer Member since: 2011
Organization: DELAWARE STATE UNIV.
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- Ease of use
- After sales service
- Value for money
"Beautiful product!"
Review date: 29 Jul 2011 | Mini-PROTEAN® TGX Stain-Free™ Precast Gels
Status:
Reviewer Member since: 2011
Organization: UNIV. OF COLORADO-DENVER
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- Ease of use
- After sales service
- Value for money
"Good product, easy to use."
Review date: 25 Jul 2011 | Mini-PROTEAN® TGX Stain-Free™ Precast Gels
Status:
Reviewer Member since: 2011
Organization: KANSAS STATE UNIVERSITY
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- Ease of use
- After sales service
- Value for money
"Great customer service. Easy to use and store. The gels are great, there is a good variety and they are easy to unlock. Great for Westerns."
Review date: 25 Jul 2011 | Mini-PROTEAN® TGX Stain-Free™ Precast Gels
Status:
Reviewer Member since: 2011
Organization: NEW YORK MEDICAL COLLEGE
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- Ease of use
- After sales service
- Value for money
"They are good, long shelf life and runs fast."
Review date: 15 Jul 2011 | Mini-PROTEAN® TGX Stain-Free™ Precast Gels
Status:
Reviewer Member since: 2011
Organization: ST BARTHOLOMEWS HOSPITAL
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- Ease of use
- After sales service
- Value for money
"Although now with increased lifespan ( 1yr versus 9 weeks before) the precast gels don't offer much value for money compared to the handmade ones."
Review date: 29 Jun 2011 | Mini-PROTEAN® TGX Stain-Free™ Precast Gels
Mini-PROTEAN® TGX Stain-Free™ Precast Gels are based on the long-shelf-life TGX (Tris-Glycine eXtended) formulation and include unique trihalo compounds that allow rapid fluorescent detection of proteins with Bio-Rad stain-free imaging systems.
Mini-PROTEAN TGX Stain-Free Precast Gels do not contain SDS. For standard denaturing electrophoresis use sample and running buffers containing SDS. To retain native protein conformation and activity these gels can be run with sample and running buffer that do not contain SDS.
Mini-PROTEAN TGX Stain-Free Gels retain Laemmli-like separation characteristics using standard sample and Tris/glycine running buffers. Protein migration patterns under non-denaturing conditions do not provide accurate molecular weight estimations.
The trihalo compounds in Mini-PROTEAN TGX Stain-Free Gels react with tryptophan residues in proteins in a UV-induced 1-minute reaction to produce fluorescence. The fluorophores remain covalently bound to the proteins throughout electrophoresis and blotting and may be subsequently visualized in gels or on PVDF membranes multiple times for validation in a western blotting workflow. The use of stain-free imaging also allows for the normalization of bands to the total protein on a blot, eliminating the use of housekeeping proteins or stripping and reprobing.
Features & Benefits:
- Run times as short as 15 minutes
- Transfer proteins in as little as 15 minutes; verify transfer to membrane with a stain-free enabled imager
- Inexpensive Laemmli buffer system, low running costs
- Gel images and complete analysis in less than 5 minutes after electrophoresis
- Comparable sensitivity to Coomassie stain
- Better reproducibility and quantitation compared to staining procedures
Applications and Uses of Mini-PROTEAN TGX Stain-Free Precast Gels:
- 1-D polyacrylamide gel electrophoresis for protein separation
- Accurate molecular weight estimation
- Robust system for complex samples
- One gel for western blotting, staining, and mass spectrometry
Product Models Information
Product Overview
Mini-PROTEAN® TGX Stain-Free™ Precast Gels
Manufacturer Bio-Rad | Available Worldwide
4.7 / 5.0 | 15 reviews