We make and supply spectrometers designed to familiarise students with the principles of fluorescence lifetime spectroscopy and in particular time correlated single photon counting (TCSPC). By monitoring the fluorescence lifetimes of emitting dyes, important information can be obtained on the molecular interactions and the micro-environment of these dyes. Proximity of quenchers, the presence of oxygen, pH level, temperature and viscosity can all influence the fluorescence lifetime. The effects of dynamic quenching and in particular the process of FRET are now widely studied in photochemistry and photobiology. A large variety of biochemical and cell-based assays are now readily available that utilise these dynamic quenching effects and which can be used with our mini- τ teaching spect...Read more
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