MicroCal iTC200 by Malvern Panalytical
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Status:
Reviewer
Member since: 2016
Organization: Boston Biomedical Inc.
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- Ease of use
- After sales service
- Value for money
Generating convincing and great data for biophysical study
Application Area: Biophysical study
"Very good product, giving us the interaction binding Kd and stoichiometry, deltaG etc...After sale customer services are also very good. It is worth having in my lab."
Review date: 22 Sep 2016 | MicroCal iTC200
Status:
Reviewer Member since: 2016
Organization: Icahn School of Medicine at Mount Sinai
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- Ease of use
- After sales service
- Value for money
This instrument is essential for my research
Application Area:Small molecule - protein interaction
"The data are very reproducible, the machine is robust and pretty easy to use. Some experiments require a little bit of optimisation but overall the sensitivity and robustness of the signal are very good"
Review date: 20 Sep 2016 | MicroCal iTC200
Status:
Reviewer Member since: 2014
Organization: Leibniz Institute of Plant Biochemistry- Molecular Signal Processing
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- Ease of use
- After sales service
- Value for money
Application Area:Protein Protein Interaction
"iTC200 micro calorimeter (MALVERN) uses minimum volume of samples compared to other old VP-ITC. Easy to handle and support from the company is very good! Binding data is also quite reproducible and reliable. Can be used both for chemical and biological experiments."
Review date: 14 Oct 2014 | MicroCal iTC200
The MicroCal iTC200 is a highly sensitive, low volume isothermal titration calorimeter for the label-free in solution study of biomolecular interactions.
It delivers direct measurement of all binding parameters in a single experiment and can analyze weak to high affinity binders, using as little as 10 µg sample. Semi-automated maintenance minimizes operator intervention and the system is upgradable to the fully automated MicroCal Auto-iTC200, making it ideal for laboratories where speed, sensitivity and the ability to accommodate higher workloads in the future are paramount.
The MicroCal iTC200 is designed for ease-of-use and exceptional sensitivity. Its wide affinity range enables analysis of weak to high affinity binders with exceptional reproducibility. Automated cleaning and syringe loading functions minimizes operator involvement while user-friendly software guides all operations for fast, accurate analysis. The system directly measures heat released or absorbed during biochemical binding events, from which it calculates binding affinity (KD), stoichiometry (n), enthalpy (ΔH), and entropy (ΔS). With minimal sample preparation and system optimization data is generated quickly and easily.
Features:
- All binding parameters (affinity, stoichiometry, enthalpy and entropy) in a single experiment.
- Quick to first results with no assay development, no labeling, no immobilization and no molecular weight limitations.
- Sensitivity to investigate any biomolecular interaction using as little as 10 µg of protein.
- Directly measures millimolar to nanomolar affinities (KDS) (10-2 to 10-9 M).
- Measures nanomolar to picomolar disassociation constants using competitive binding techniques (10-9to 10-12M).
- Experimental design wizards and automated data analysis for fast and reliable analysis.
- Coin shaped cell optimizes sample mixing. Small cell volume – less sample consumption.
- Nonreactive Hastelloy for chemical resistance and compatibility with biological samples.
- Semi-automated cell filling, injection and cell cleaning.
- Compatible with non-aqueous solvents.
- User friendly software for fast and accurate analysis.
- Upgradable to fully automated MicroCal Auto-iTC200.
Applications:
- Characterizing biomolecular interactions, to: Confirm binding and activity
- Determine stoichiometry and thermodynamic parameters
- Studying the interaction of any two biomolecules including: Proteins, nucleic acids, lipids, drugs and inhibitors
- Structural biology and structure-activity relationships
- Drug discovery for: Hit validation and characterization
- Lead optimization Mechanism of action
Product Overview
