Average Rating: 4.3
1 Scientist has reviewed this product
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"So incredibly easy to link GOx, it has reproducible results and the shipping time is outstanding. The amount of time we spend ordering and receiving then preparing this reagent is negligible. "Review date:
31 Jan 2014 | Lightning-Link™ Glucose Oxidase (GOx) kit
Say goodbye to secondary antibodies – Go direct using the World’s Easiest DIY Antibody Labeling Kit - Lightning-Link™!
• Eliminates need for secondary antibodies
• Reduces the number of wash steps
• Ideal for multi-protein analysis – with over 40 available labels
• Very easy to use
• 100% antibody recovery
The DIY Lightning-Link™ kit allows you to label an antibody with minimal hands-on time - less than 30 seconds. Simply add your antibody solution to a tube of Lightning-Link™ mix and the label becomes covalently attached to your antibody in a gentle controlled process. Lightning-Link is the easiest and quickest method available for antibody conjugation.
Direct antibodies labelling greatly simplifies immunodetection techniques. Without the problems of crossover and/or non-specific binding from secondary reagents it is far easier to obtain high quality, pioneering results in techniques such as flow cytometry, ELISA, Western blotting and immunohistochemistry. A reduction in the number of incubation and wash steps also saves you time and money.
The antibody conjugate:
Glucose oxidase is an enzyme produced and purified from Aspergillus niger which catalyses the oxidation of glucose with the release of hydrogen peroxide. It's molecular weight is 80kDa, but it exists as a dimer of 160kDa Measurement of glucose by GOx is used in the food industry, in fermentation and, most importantly, as the basis of biosensors for the diagnosis of diabetes.