Say goodbye to secondary antibodies – Go direct using the World’s Easiest DIY Antibody Labeling Kit - Lightning-Link™!
• Eliminates need for secondary antibodies • Reduces the number of wash steps • Ideal for multi-protein analysis – with over 40 available labels • Very easy to use • 100% antibody recovery
The DIY Lightning-Link™ kit allows you to label an antibody with minimal hands-on time - less than 30 seconds. Simply add your antibody solution to a tube of Lightning-Link™ mix and the label becomes covalently attached to your antibody in a gentle controlled process. Lightning-Link is the easiest and quickest method available for antibody conjugation.
Direct antibodies labeling greatly simplifies immunodetection techniques. Without the problems of crossover and/or non-specific binding from secondary reagents it is far easier to obtain high quality, pioneering results in techniques such as flow cytometry, ELISA, Western blotting and immunohistochemistry. A reduction in the number of incubation and wash steps also saves you time and money.
The antibody conjugate:
Cyanine Dye 5 (Cy5) has a strong absorption peak at 643nm and a secondary absorption peak at 605nm. The maximum emission peak is at 667nm, with an excitation coefficient of 2.5 x105 cm-1 M-1. Cy5 can be used as an alternative to AlexaFluor 647, and the Phycobiliprotein Allophycocyanin.