Lightning-Link™ Atto 590 kit
Say goodbye to secondary antibodies – Go direct using the World’s Easiest DIY Antibody Labeling Kit - Lightning-Link™!
• Eliminates need for secondary antibodies
• Reduces the number of wash steps
• Ideal for multi-protein analysis – with over 40 available labels
• Very easy to use
• 100% antibody recovery
The DIY Lightning-Link™ kit allows you to label an antibody with minimal hands-on time - less than 30 seconds. Simply add your antibody solution to a tube of Lightning-Link™ mix and the label becomes covalently attached to your antibody in a gentle controlled process. Lightning-Link is the easiest and quickest method available for antibody conjugation.
Direct antibodies labelling greatly simplifies immunodetection techniques. Without the problems of crossover and/or non-specific binding from secondary reagents it is far easier to obtain high quality, pioneering results in techniques such as flow cytometry, ELISA, Western blotting and immunohistochemistry. A reduction in the number of incubation and wash steps also saves you time and money.
The antibody conjugate:
Atto590 belongs to the Rhodamine class of fluorescent labels of new generation. It has a strong absorption with its maximum at 600nm (and Extinction Coefficient of 1.2 x 105 M-1 cm-1), and a high fluorescence quantum yield, with the maximum emission at 630nm. Atto590 can be used as a substitute dye for Alexa Fluor 594, Carboxynapthofluorescein and DY-610.