Say goodbye to secondary antibodies – Go direct using the World’s Easiest DIY Antibody Labeling Kit - Lightning-Link™! • Eliminates need for secondary antibodies • Reduces the number of wash step...read more
Say goodbye to secondary antibodies – Go direct using the World’s Easiest DIY Antibody Labeling Kit - Lightning-Link™!
• Eliminates need for secondary antibodies • Reduces the number of wash steps • Ideal for multi-protein analysis – with over 40 available labels • Very easy to use • 100% antibody recovery
The DIY Lightning-Link™ kit allows you to label an antibody with minimal hands-on time - less than 30 seconds. Simply add your antibody solution to a tube of Lightning-Link™ mix and the label becomes covalently attached to your antibody in a gentle controlled process. Lightning-Link is the easiest and quickest method available for antibody conjugation.
Direct antibody labelling greatly simplifies immunodetection techniques. Without the problems of crossover and/or non-specific binding from secondary reagents it is far easier to obtain high quality, pioneering results in techniques such as flow cytometry, ELISA, Western blotting and immunohistochemistry. A reduction in the number of incubation and wash steps also saves you time and money.
The anitbody conjugate: AMCA (aminomethylcoumarin acetate) is a blue fluorescent dye commonly used in fluorescence microscopy, arrays and in-situ hybridisation. It has an excitation wavelength of 345-350nm and an emission wavelength of 440-460nm. Unlike standard AMCA labeling procedures, where a large molar excess of AMCA is employed, the Lightning-Link AMCA kit combines a low molar ratio of AMCA:antibody and high conjugation efficiency to eliminate the need for purification steps.