LightCycler® 480 Real-Time PCR System

LightCycler® 480 Real-Time PCR System
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by Roche Applied Science - a member of the Roche Group
 4.3/5.0 (31 reviews)

The Roche LightCycler 480 System - Another breakthrough in real-time PCR analysis of gene expression and melting curve-based mutation analysis. The Roche LightCycler 480 RT-PCR System combines amazing speed with exceptional accuracy and will meet the needs of a broad range of scientific applications in genomics research including array validation, gene-knockdown studies, and SNP analysis. This...read more

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Reviews

AVERAGE RATING: 4.3

31 scientists have reviewed this product

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  • 4 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY

Jianming Xu


Status:


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Member since 2013

Organization:
German Cancer Research Center
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RATING: 4.0

  • 4 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 22 Apr 2014

Application Area: Real-Time PCR
LightCycler® 480 Real-Time PCR System

"This is a good machine for Real-Time PCR. "

Read 31 reviews

Emiel Janssen


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Member since 2009

Organization:
Stavanger University Hospital
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RATING: 4.7

  • 5 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 15 Apr 2014

Application Area: Cancer Research
LightCycler® 480 Real-Time PCR System

"It is a stable and good instrument for real time PCR, we use it for both diagnostics and research. It’s reliable and easy to learn to users. Both 96 -and 384 wells blocks can be used and are easy to exchange. "

Dhara Patel


Status:


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Member since 2013

Organization:
Washington University in St. Louis
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RATING: 4.7

  • 5 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 10 Feb 2014

Application Area: qPCR
LightCycler® 480 Real-Time PCR System

"We use the lightcycler 480. It is a great instrument and software. The software allows data analysis while reading."

Anand Patel


Status:


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Member since 2011

Organization:
Washington University in St. Louis
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RATING: 5.0

  • 5 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY




Review date: 10 Feb 2014

Application Area: qPCR
LightCycler® 480 Real-Time PCR System

"It is much better value for the money than the comparable instrument from Life Tech and better field scientist support. It is very robust and easily integrated with automation. "

CECILIA FERNANDEZ


Status:


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Member since 2013

Organization:
PREDICTIVE BIOSCIENCES
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RATING: 3.7

  • 4 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 3 out of 5VALUE FOR MONEY




Review date: 06 Aug 2013

Application Area: diagnostics
LightCycler® 480 Real-Time PCR System

"Good reproducibility and accuracy from a quick working instrument. It is very easy to perform optimization experiments and the software is easy to use."

Meir Lahav


Status:


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Member since 2013

Organization:
Beilinson Hosp. Tel Aviv Univ.
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RATING: 5.0

  • 5 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY




Review date: 03 Jun 2013

Application Area: molecular biology
LightCycler® 480 Real-Time PCR System

"This is an easy to use and very reliable instrument. In addition there is excellent support from the company. We found it very helpful in our lab."

Meir Lahav


Status:


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Member since 2013

Organization:
Beilinson Hosp. Tel Aviv Univ.
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RATING: 5.0

  • 5 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY




Review date: 03 Jun 2013

Application Area: molecular biology
LightCycler® 480 Real-Time PCR System

"Easy to use, with excellent support. Very reliable and handy.  Easy to develop new assays for."

Andre White


Status:


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Member since 2012

Organization:
UC Irvine
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RATING: 4.0

  • 4 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 26 Nov 2012

Application Area: PCR Molecular Biology
LightCycler® 480 Real-Time PCR System

"Simple sample loading and analysis. Also, Roche support staff are helpful."

JIHUI DU


Status:


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Member since 2010

Job Title:
Research Scientist / Senior / PI
Discipline:
Molecular biology
Work Field:
Hospital Laboratory
Organization:
SHENZHEN NANSHAN HOSPITAL
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RATING: 4.3

  • 5 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 13 Jun 2012

Application Area: Clinical gene diagnostics
LightCycler® 480 Real-Time PCR System

"Easy to use and fast to run a PCR reaction. Gives high accuracy and it has HRM function to use in gene mutation detection."

KENNETH TO


Status:


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Member since 2010

Organization:
THE CHINESE UNIVERSITY OF HONG KONG
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RATING: 4.3

  • 5 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 13 Jun 2012

Application Area: Real time PCR
LightCycler® 480 Real-Time PCR System

"Easy to use and the software for analysis is convenient."

Stan Lightfoot


Status:


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Member since 2009

Organization:
OUHSC
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RATING: 4.6

  • 5 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY




Review date: 31 May 2012

LightCycler® 480 Real-Time PCR System

"Excellent in our lab, efficient and easy to use."

Marc Weinberg


Status:


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Member since 2011

Organization:
University of North Carolina A
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RATING: 5.0

  • 5 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY




Review date: 05 Dec 2011

LightCycler® 480 Real-Time PCR System

"Fantastic and easy to use. It can go from start to finish within minutes. Flexible to use with a variety of PCR products. Storage of data allows for safety (no data loss if you choose to analyze in multiple ways). However, with the capacity to hold 96 wells I found the machine to be a little on the large side. The service team offers extensive training before and after you purchase."

Antje Bleumke


Status:


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Member since 2011

Organization:
Hamburg University
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RATING: 4.3

  • 4 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 03 Nov 2011

LightCycler® 480 Real-Time PCR System

"Works best with Roche Mastermixes. It's easy to handle, only one button to open. However programming is quite difficult, not like a normal PCR cycler."

Claudius Stordeur


Status:


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Member since 2011

Organization:
MLV Halle
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RATING: 3.6

  • 4 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 3 out of 5VALUE FOR MONEY




Review date: 28 Oct 2011

LightCycler® 480 Real-Time PCR System

"A fast and reliable system. A very low number of molecules detectable. The system can be used for DNA quantification. The number of copies affect the success of the process."

Anne Frentzen


Status:


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Member since 2011

Organization:
Twincore
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RATING: 4.3

  • 4 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 20 Oct 2011

LightCycler® 480 Real-Time PCR System

"Equipment and manual are easy to understand and apply, 96 well plate allows analysis of many samples. This system can be used for many applications including SYBR green assays, master hydrolysis probes, genotyping and melling curves. They provide good service in case of any hardware problems."

ANA LUISA CORREIA


Status:


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Member since 2011

Organization:
LAWRENCE BERKELEY NAT'L LAB.
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RATING: 4.6

  • 5 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 20 Jul 2011

LightCycler® 480 Real-Time PCR System

"Very accurate and user friendly."

JESSICA POISSON


Status:


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Member since 2011

Organization:
UNIV OF NORTH CAROLINA HOSPITALS
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RATING: 4.3

  • 4 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 29 Jun 2011

LightCycler® 480 Real-Time PCR System

"A fast and very easy-to-use PCR. The closed system is excellent and it is small enough for the bench top."

ABDUL SIRAJ


Status:


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Member since 2011

Organization:
KING FAISAL SPECIALIST HOSPITAL
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RATING: 3.6

  • 4 out of 5EASE OF USE
  • 3 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 29 Jun 2011

LightCycler® 480 Real-Time PCR System

"An excellent system for real-time PCR."

Randy Scott


Status:


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Member since 2011

Organization:
BioExpress
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RATING: 5.0

  • 5 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY




Review date: 16 Mar 2011

LightCycler® 480 Real-Time PCR System

Mylan Pho


Status:


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Member since 2011

Organization:
Genomic Health
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RATING: 4.0

  • 4 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 04 Mar 2011

LightCycler® 480 Real-Time PCR System

"Very reliable."

Holly Green


Status:


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Member since 2010

Organization:
Dept. Anatomy And Neuroscience
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RATING: 4.3

  • 4 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 24 Nov 2010

LightCycler® 480 Real-Time PCR System

"Quick and easy to use, good for high through put."

Todd Dehm


Status:


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Member since 2010

Organization:
The Jackson Laboratory
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RATING: 4.0

  • 4 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 18 Feb 2010

LightCycler® 480 Real-Time PCR System

"The system works really well for melt curve and real-time PCR."

Mark Herrmann


Status:


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Member since 2010

Organization:
ARUP LABORATORIES
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RATING: 5.0

  • 5 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY




Review date: 15 Feb 2010

LightCycler® 480 Real-Time PCR System

"Versatile instrumentation for genetic analysis."

Pedro Lee


Status:


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Member since 2010

Organization:
Univ California-San Diego
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RATING: 4.3

  • 4 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 20 Jan 2010

LightCycler® 480 Real-Time PCR System

"A fast, easy-to-use and accurate machine."

Christine Buerk


Status:


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Member since 2008

Organization:
Muva Kempten
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RATING: 4.0

  • 4 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 20 Jun 2008

LightCycler® 480 Real-Time PCR System

"An excellent system."

Yin Leng Lee


Status:


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Member since 2007

Organization:
Mount Sinai School of Medicine
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RATING: 4.3

  • 5 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 19 Oct 2007

LightCycler® 480 Real-Time PCR System

Jaime Agostino


Status:


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Member since 2007

Organization:
Wadsworth Center
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RATING: 4.0

  • 4 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 12 May 2007

LightCycler® 480 Real-Time PCR System

Hong Wu


Status:


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Member since 2007

Organization:
Wadsworth Center
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RATING: 4.0

  • 4 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 12 May 2007

LightCycler® 480 Real-Time PCR System

Hamidou


Status:


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Member since 2006

Organization:
Tenon
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RATING: 4.6

  • 5 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 27 Apr 2006

LightCycler® 480 Real-Time PCR System

"Very easy to use."

Bouche Naki


Status:


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Member since 2006

Organization:
ICO Pal
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RATING: 4.0

  • 4 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 21 Apr 2006

LightCycler® 480 Real-Time PCR System

"Easy to use."

Daniel Filho


Status:


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Member since 2006

Organization:
Universidade Federal do Acre
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RATING: 4.0

  • 4 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 09 Feb 2006

LightCycler® 480 Real-Time PCR System

"A very good product for Real-time PCR."

Description

 

The Roche LightCycler 480 System - Another breakthrough in real-time PCR analysis of gene expression and melting curve-based mutation analysis.

The Roche LightCycler 480 RT-PCR System combines amazing speed with exceptional accuracy and will meet the needs of a broad range of scientific applications in genomics research including array validation, gene-knockdown studies, and SNP analysis.

This Roche Light Cycler PCR supports all current probe formats and applications and its built-in versatility allows adaptation to future technological advances in genomics research.

Customer benefits from the Roche LightCycler® 480 System:

  • Speed – rapid temperature control for superb specificity and yield.
    The LightCycler 480 PCR System utilizes a novel block technology to allow the rapid heating and cooling required to complete a 40-cycle, 384-well quantitative PCR run in less than 40 minutes and leads to extraordinary well-to-well temperature homogeneity and maximized inter-well, inter-cycle reproducibility.
  • Accuracy – unbiased conditions for unambiguous results
    Special arrangement of optical components ensures the uniform collection of signals across the plate and makes analysis independent of sample position or differences in reaction volume.
  • Versatility – utmost flexibility for maximum efficiency
    Five excitation and six emission filters of the Roche LightCycler allow you to work with the assay format of your choice, including SYBR Green I, hydrolysis probes, probes, probes, Molecular Beacons, and others.
  • High-performance optical system from the LightCycler 480
    Fluorescence excitation by a high-intensity xenon lamp, and a specially developed signal-collection system, result in maximal sensitivity for any assay format in all channels.

Additional Features of the LightCycler 480 PCR System:

  • Customizable modular system concept
    Building on the principles of the well-established, performance-proven LightCycler® Software 4.0, the Roche LightCycler® 480 platform comes equipped with modules for run control, Tm calling, and absolute quantification. Further customize your system to your specific needs by adding separately offered advanced analysis modules.
  • Especially developed reagents
    A tailor-made hot-start PCR enzyme master mix is available for each type of LightCycler® 480 application. Built on Roche Diagnostics’ performance proven FastStart technology, these reagents ensure optimal reproducibility and high dynamic ranges.
  • Perfect integration into laboratory workflow
    Compatibility for automated sample-preparation, automated loading and connectivity to laboratory information management systems (LIMS) for integrated workflow monitoring offer uncompromising adaptability for a perfect fit.

Product Overview

LightCycler® 480 Real-Time PCR System by Roche Applied Science - a member of the Roche Group
LightCycler® 480 Real-Time PCR System




  • 4out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY





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