LightCycler® 480 Real-Time PCR System

4.3
/
5.0
  |  36 reviews


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Average Rating: 4.3
36 Scientists have reviewed this product

4 out of 5
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money


It is a stable and good instrument for real time PCR
Rating: 5.0

  • Application Area: THE real time machine - forget the others

"We also use it, alongside a BioRad CFX96 and an ABI 7500. Out of the three, I think the Roche has the best sensitivity, and it also allows you to buy a 384 well block that can be easily interchanged if you want to add high throughput capability. However, the software (or at least the version that we have) is not particularly quick, cannot be loaded on a personal computer, and doesn't have many options for ways to export the data. And when we were interested in expanding into using HRM, we had to cough up a lot of cash to get the upgraded software module which we haven't found to be particularly useful. In contrast, the BioRad CFX is our go-to machine for most 96 well applications. The machine is good, consistent, has never needed maintenance (even though it's often used 6x per day), and has faster ramp times. But the real boon is the software. The basic software is simple to use, can be loaded on everyone's computer for offline analysis, and offers even the difficult normalization options (like normalization to the geometric mean of several reference genes, or ways to normalize across several plates in a multi-plate experiment). It even offers plate wide melt calibration so it gives MUCH more consistent results for anything reliant on melt (like HRM). On balance, the Roche instrument doesn't seem anywhere near superior enough to the BioRad to justify the terrible software. I'd highly recommend taking a look at the BioRad CFX96. It's the one that gets the most use around here!"

Review date: 24 Mar 2016 | LightCycler® 480 Real-Time PCR System
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: Applied Photophyscis



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Unmatchable results with LNA probes & an innovation for gene expression studies.
Rating: 4.7

  • Application Area:Mutation detection & gene quantification

"LightCycler 480 II System is a highly innovative instrument which uses specific optics for having the entire plate analysis simultaneously unlike well-to-well scanning systems producing high errors. LNA probes is a patented technology and offers exceptional specificity. LightCycler 480 II System is industry gold standard in terms of accuracy, reproducibility and repeatability of its results. "

Review date: 16 Dec 2015 | LightCycler® 480 Real-Time PCR System
  • Status:

    Reviewer

  • Member since: 2011

  • Organization: Washington University in St. Louis



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rating: 5.0

  • Application Area:qPCR

"It is much better value for the money than the comparable instrument from LifeTech and better field scientist support. It is very robust and easily integrated with automation. "

Review date: 12 Oct 2015 | LightCycler® 480 Real-Time PCR System
  • Status:

    Advanced Reviewer

  • Member since: 2013

  • Organization: GEM Tox Consultants & Labs, Inc.



  • Ease of use
    3 out of 5
    After sales service
    3 out of 5
    Value for money
    3 out of 5
Rating: 3.0

  • Application Area:Analyze Gene and microRNA Expression

"The Roche LightCycler 480 is a PCR instrument that uses either 96 or 384 well plates to carry out automatic measurements on gene expression (or microRNA expression). The instrument can be easily used. It uses melting temperatures to determine the quality and purity of the template. The machine can be serviced by Roche in Indianapolis, IN, and is reasonably priced. I am a fan of Roche and I recommend this product for accurate measurement of gene or micRNA expression."

Review date: 06 Aug 2015 | LightCycler® 480 Real-Time PCR System
  • Status:

    Reviewer

  • Member since: 2011

  • Organization: Faculty of Pharmacy Cairo University



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Rating: 4.3
"It combines amazing speed with exceptional accuracy which will provides the needs of a broad range of scientific applications in genomics research including array validation, gene-knockdown studies, and SNP analysis. Another benefit is the good reproducibility and accuracy from a quick working instrument. It is very easy to perform optimization experiments and the software is easy to use. moreover, The LightCycler 480 PCR System utilizes a novel block technology to allow the rapid heating and cooling required to complete a 40-cycle, 384-well quantitative PCR run in less than 40 minutes and leads to extraordinary well-to-well temperature homogeneity and maximized inter-well, inter-cycle reproducibility. Finally, we suggest this system because the compatibility for automated sample-preparation, automated loading and connectivity to laboratory information management systems (LIMS) for integrated workflow monitoring offer uncompromising adaptability for a perfect fit."

Review date: 30 Jan 2015 | LightCycler® 480 Real-Time PCR System


  • Status:

    Advanced Reviewer

  • Member since: 2013

  • Organization: German Cancer Research Center



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Rating: 4.0

  • Application Area:Real-Time PCR

"This is a good machine for real-time PCR. "

Review date: 05 Nov 2014 | LightCycler® 480 Real-Time PCR System
  • Status:

    Advanced Reviewer

  • Member since: 2008

  • Organization: Zanjan University of Medical Sciences



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Rating: 4.3

  • Application Area:Relative quantification gene expression

"This device is fully sophisticated, GLP based and valuable for work."

Review date: 27 Oct 2014 | LightCycler® 480 Real-Time PCR System
  • Status:

    Reviewer

  • Member since: 2009

  • Organization: Stavanger University Hospital



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Rating: 4.7

  • Application Area:Cancer Research

"It is a stable and good instrument for real time PCR, we use it for both diagnostics and research. It’s reliable and easy to learn to users. Both 96 -and 384 wells blocks can be used and are easy to exchange. "

Review date: 15 Apr 2014 | LightCycler® 480 Real-Time PCR System
  • Status:

    Reviewer

  • Member since: 2013

  • Organization: Washington University in St. Louis



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Rating: 4.7

  • Application Area:qPCR

"We use the lightcycler 480. It is a great instrument and software. The software allows data analysis while reading."

Review date: 10 Feb 2014 | LightCycler® 480 Real-Time PCR System
  • Status:

    Reviewer

  • Member since: 2013

  • Organization: PREDICTIVE BIOSCIENCES



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    3 out of 5
Rating: 3.7

  • Application Area:diagnostics

"Good reproducibility and accuracy from a quick working instrument. It is very easy to perform optimization experiments and the software is easy to use."

Review date: 06 Aug 2013 | LightCycler® 480 Real-Time PCR System
  • Status:

    Reviewer

  • Member since: 2013

  • Organization: Beilinson Hosp. Tel Aviv Univ.



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rating: 5.0

  • Application Area:molecular biology

"This is an easy to use and very reliable instrument. In addition there is excellent support from the company. We found it very helpful in our lab."

Review date: 03 Jun 2013 | LightCycler® 480 Real-Time PCR System
  • Status:

    Reviewer

  • Member since: 2013

  • Organization: Beilinson Hosp. Tel Aviv Univ.



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rating: 5.0

  • Application Area:molecular biology

"Easy to use, with excellent support. Very reliable and handy.  Easy to develop new assays for."

Review date: 03 Jun 2013 | LightCycler® 480 Real-Time PCR System
  • Status:

    Reviewer

  • Member since: 2012

  • Organization: UC Irvine



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Rating: 4.0

  • Application Area:PCR Molecular Biology

"Simple sample loading and analysis. Also, Roche support staff are helpful."

Review date: 26 Nov 2012 | LightCycler® 480 Real-Time PCR System
  • Status:

    Reviewer

  • Member since: 2010

  • Organization: SHENZHEN NANSHAN HOSPITAL



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Rating: 4.3

  • Application Area:Clinical gene diagnostics

"Easy to use and fast to run a PCR reaction. Gives high accuracy and it has HRM function to use in gene mutation detection."

Review date: 13 Jun 2012 | LightCycler® 480 Real-Time PCR System
  • Status:

    Reviewer

  • Member since: 2010

  • Organization: THE CHINESE UNIVERSITY OF HONG KONG



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Rating: 4.3

  • Application Area:Real time PCR

"Easy to use and the software for analysis is convenient."

Review date: 13 Jun 2012 | LightCycler® 480 Real-Time PCR System
  • Status:

    Reviewer

  • Member since: 2009

  • Organization: OUHSC



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Rating: 4.7
"Excellent in our lab, efficient and easy to use."

Review date: 31 May 2012 | LightCycler® 480 Real-Time PCR System
  • Status:

    Reviewer

  • Member since: 2011

  • Organization: University of North Carolina A



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rating: 5.0
"Fantastic and easy to use. It can go from start to finish within minutes. Flexible to use with a variety of PCR products. Storage of data allows for safety (no data loss if you choose to analyze in multiple ways). However, with the capacity to hold 96 wells I found the machine to be a little on the large side. The service team offers extensive training before and after you purchase."

Review date: 05 Dec 2011 | LightCycler® 480 Real-Time PCR System
  • Status:

    Reviewer

  • Member since: 2011

  • Organization: Hamburg University



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Rating: 4.3
"Works best with Roche Mastermixes. It's easy to handle, only one button to open. However programming is quite difficult, not like a normal PCR cycler."

Review date: 03 Nov 2011 | LightCycler® 480 Real-Time PCR System
  • Status:

    Reviewer

  • Member since: 2011

  • Organization: MLV Halle



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    3 out of 5
Rating: 3.7
"A fast and reliable system. A very low number of molecules detectable. The system can be used for DNA quantification. The number of copies affect the success of the process."

Review date: 28 Oct 2011 | LightCycler® 480 Real-Time PCR System
  • Status:

    Reviewer

  • Member since: 2011

  • Organization: Twincore



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Rating: 4.3
"Equipment and manual are easy to understand and apply, 96 well plate allows analysis of many samples. This system can be used for many applications including SYBR green assays, master hydrolysis probes, genotyping and melling curves. They provide good service in case of any hardware problems."

Review date: 20 Oct 2011 | LightCycler® 480 Real-Time PCR System
  • Status:

    Reviewer

  • Member since: 2011

  • Organization: LAWRENCE BERKELEY NAT'L LAB.



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Rating: 4.7
"Very accurate and user friendly."

Review date: 20 Jul 2011 | LightCycler® 480 Real-Time PCR System
  • Status:

    Reviewer

  • Member since: 2011

  • Organization: UNIV OF NORTH CAROLINA HOSPITALS



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Rating: 4.3
"A fast and very easy-to-use PCR. The closed system is excellent and it is small enough for the bench top."

Review date: 29 Jun 2011 | LightCycler® 480 Real-Time PCR System
  • Status:

    Reviewer

  • Member since: 2011

  • Organization: KING FAISAL SPECIALIST HOSPITAL



  • Ease of use
    4 out of 5
    After sales service
    3 out of 5
    Value for money
    4 out of 5
Rating: 3.7
"An excellent system for real-time PCR."

Review date: 29 Jun 2011 | LightCycler® 480 Real-Time PCR System
  • Status:

    Reviewer

  • Member since: 2011

  • Organization: BioExpress



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rating: 5.0

Review date: 16 Mar 2011 | LightCycler® 480 Real-Time PCR System
  • Status:

    Reviewer

  • Member since: 2011

  • Organization: Genomic Health



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Rating: 4.0
"Very reliable."

Review date: 04 Mar 2011 | LightCycler® 480 Real-Time PCR System
  • Status:

    Reviewer

  • Member since: 2010

  • Organization: Dept. Anatomy And Neuroscience



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Rating: 4.3
"Quick and easy to use, good for high through put."

Review date: 24 Nov 2010 | LightCycler® 480 Real-Time PCR System
  • Status:

    Reviewer

  • Member since: 2010

  • Organization: The Jackson Laboratory



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Rating: 4.0
"The system works really well for melt curve and real-time PCR."

Review date: 18 Feb 2010 | LightCycler® 480 Real-Time PCR System
  • Status:

    Reviewer

  • Member since: 2010

  • Organization: ARUP LABORATORIES



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rating: 5.0
"Versatile instrumentation for genetic analysis."

Review date: 15 Feb 2010 | LightCycler® 480 Real-Time PCR System
  • Status:

    Reviewer

  • Member since: 2010

  • Organization: Univ California-San Diego



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Rating: 4.3
"A fast, easy-to-use and accurate machine."

Review date: 20 Jan 2010 | LightCycler® 480 Real-Time PCR System
  • Status:

    Reviewer

  • Member since: 2008

  • Organization: Muva Kempten



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Rating: 4.0
"An excellent system."

Review date: 20 Jun 2008 | LightCycler® 480 Real-Time PCR System
  • Status:

    Reviewer

  • Member since: 2007

  • Organization: Mount Sinai School of Medicine



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Rating: 4.3

Review date: 19 Oct 2007 | LightCycler® 480 Real-Time PCR System
  • Status:

    Reviewer

  • Member since: 2007

  • Organization: Wadsworth Center



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Rating: 4.0

Review date: 12 May 2007 | LightCycler® 480 Real-Time PCR System
  • Status:

    Reviewer

  • Member since: 2007

  • Organization: Wadsworth Center



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Rating: 4.0

Review date: 12 May 2007 | LightCycler® 480 Real-Time PCR System
  • Status:

    Reviewer

  • Member since: 2006

  • Organization: Tenon



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Rating: 4.7
"Very easy to use."

Review date: 27 Apr 2006 | LightCycler® 480 Real-Time PCR System
  • Status:

    Reviewer

  • Member since: 2006

  • Organization: ICO Pal



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Rating: 4.0
"Easy to use."

Review date: 21 Apr 2006 | LightCycler® 480 Real-Time PCR System
  • Status:

    Advanced Reviewer

  • Member since: 2006

  • Organization: Universidade Federal do Acre



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Rating: 4.0
"A very good product for Real-time PCR."

Review date: 09 Feb 2006 | LightCycler® 480 Real-Time PCR System
The Roche LightCycler 480 System - Another breakthrough in real-time PCR analysis of gene expression and melting curve-based mutation analysis.

The Roche LightCycler 480 RT-PCR System combines amazing speed with exceptional accuracy and will meet the needs of a broad range of scientific applications in genomics research including array validation, gene-knockdown studies, and SNP analysis.

This Roche Light Cycler PCR supports all current probe formats and applications and its built-in versatility allows adaptation to future technological advances in genomics research.

Customer benefits from the Roche LightCycler® 480 System:

  • Speed – rapid temperature control for superb specificity and yield.
    The LightCycler 480 PCR System utilizes a novel block technology to allow the rapid heating and cooling required to complete a 40-cycle, 384-well quantitative PCR run in less than 40 minutes and leads to extraordinary well-to-well temperature homogeneity and maximized inter-well, inter-cycle reproducibility.
  • Accuracy – unbiased conditions for unambiguous results
    Special arrangement of optical components ensures the uniform collection of signals across the plate and makes analysis independent of sample position or differences in reaction volume.
  • Versatility – utmost flexibility for maximum efficiency
    Five excitation and six emission filters of the Roche LightCycler allow you to work with the assay format of your choice, including SYBR Green I, hydrolysis probes, probes, probes, Molecular Beacons, and others.
  • High-performance optical system from the LightCycler 480
    Fluorescence excitation by a high-intensity xenon lamp, and a specially developed signal-collection system, result in maximal sensitivity for any assay format in all channels.

Additional Features of the LightCycler 480 PCR System:

  • Customizable modular system concept
    Building on the principles of the well-established, performance-proven LightCycler® Software 4.0, the Roche LightCycler® 480 platform comes equipped with modules for run control, Tm calling, and absolute quantification. Further customize your system to your specific needs by adding separately offered advanced analysis modules.
  • Especially developed reagents
    A tailor-made hot-start PCR enzyme master mix is available for each type of LightCycler® 480 application. Built on Roche Diagnostics’ performance proven FastStart technology, these reagents ensure optimal reproducibility and high dynamic ranges.
  • Perfect integration into laboratory workflow
    Compatibility for automated sample-preparation, automated loading and connectivity to laboratory information management systems (LIMS) for integrated workflow monitoring offer uncompromising adaptability for a perfect fit.

Product Overview

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LightCycler® 480 Real-Time PCR System

Manufacturer Roche Applied Science - a member of the Roche Group

4.3 / 5.0 | 36 reviews