LIFA-X - Long Lifetime Phosphorescence Imaging
The newest addition to the LIFA product line is the LIFA-X. LIFA stands for Lambert Instruments Fluorescence lifetime imaging Attachment. The LIFA-X is for long lifetime imaging microscopy of lifetimes from 0.1 ns up to 1 ms (phosphorescence). The ‘X’ represents the extended range of lifetimes that can be resolved when compared to the LIFA (0.1 ns – 300 ns). The LIFA-X can be used for, e.g., measuring oxygen concentrations using optodes or reactive oxygen species in cells.
The LIFA-X is based on the LIFA (Lambert Instruments Fluorescence lifetime imaging Attachment) for FLIM -Fluorescence Lifetime Imaging Microscopy and is designed in such a way that it can be retro fitted onto an existing LIFA system. Therefore, it has the same basic features and advantages as the “Lambert Instruments Fluorescence lifetime imaging Attachment”:
• It can be attached to any widefield fluorescence microscope
• Works in the homodyne frequency domain
• Is easy to install (within 1 hour) and to operate>
• Does not require an optical bench with laser.
The LIFA system has been judged "easy and highly quantitative".