Lentil Lectin Sepharose 4B

Lentil Lectin Sepharose media is used for purification of a number of glycoproteins and other carbohydrate containing molecules. The lentil lectin ligand has more equal affinity for α-D-mannose and α-D-glucose compared to Con A. Lentil lectin is a metalloprotein, containing Ca2₊ and Mn2₊, which binds reversibly to polysaccharides and glycoconjugates containing glucose or mannose type sugars. Lentil Lectin Sepharose 4B is a generally applicable group specific adsorbent and is routinely used in the preparation and purification of a number of glycoproteins and carbohydrate containing molecules. Features: Binds molecules that contain α-D-mannose, α-D-glucose, and sterically related residues Retains binding in 1% deoxycholate and is especially valuable for purification of detergent-...Read more

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Average Rating: 5.0

Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
5 out of 5
Rating: 5.0
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
5 out of 5
  • Review date: 29 May 2014

  • Application Area: Protein Purification

  • Lentil Lectin Sepharose 4B

"Lentil Lectin Sepharose 4B is an affinity resin produced by GE HealthCare Life Sciences. This resin is used for purification of a number of glycoproteins. The resin has high affinity for glycoproteins and can binds 16 milligrams of protein per ml of resins. I recommend this resin for purification of glycoproteins."

Description

Lentil Lectin Sepharose media is used for purification of a number of glycoproteins and other carbohydrate containing molecules. The lentil lectin ligand has more equal affinity for α-D-mannose and α-D-glucose compared to Con A.

Lentil lectin is a metalloprotein, containing Ca2₊ and Mn2₊, which binds reversibly to polysaccharides and glycoconjugates containing glucose or mannose type sugars. Lentil Lectin Sepharose 4B is a generally applicable group specific adsorbent and is routinely used in the preparation and purification of a number of glycoproteins and carbohydrate containing molecules.

Features:

  • Binds molecules that contain α-D-mannose, α-D-glucose, and sterically related residues
  • Retains binding in 1% deoxycholate and is especially valuable for purification of detergent-solubilized membrane proteins
  • Lentil lectin is a metalloprotein, containing Ca2₊ and Mn2₊, which binds reversibly to polysaccharides and glycoconjugates containing glucose or mannose type sugars
  • Compared to Con A, also for glycoprotein purification, Lentil Lectin distinguishes less sharply between glucosyl and mannosyl

Product Overview

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Lentil Lectin Sepharose 4B

by GE Healthcare

5.0/5.0 (1 reviews)
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
5 out of 5






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