LANCE® Ultra KinaSelect™ Ser/Thr Kit, 5 X 250 assay points


PerkinElmer, Inc.

The LANCE® Ultra KinaSelect™ Ser/Thr kit is intended for selecting the optimal peptide substrate for serine and threonine (Ser/Thr) kinases. Kinase activity is measured in a LANCE® time-resolved...read more

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Description

 

The LANCE® Ultra KinaSelect™ Ser/Thr kit is intended for selecting the optimal peptide substrate for serine and threonine (Ser/Thr) kinases.

Kinase activity is measured in a LANCE® time-resolved fluorescence resonance energy transfer (TR-FRET) assay using five different ULight-labeled peptide substrates with their corresponding europium (Eu)-labeled anti-phospho-antibodies. Substrate/antibody pairs giving the best performance can then be used for further assay development and optimization. The five ULight-labeled peptides and Eu-labeled antibodies selected for the KinaSelect kit were found to generate signal with over 80% of a panel of 184 Ser/Thr kinases.

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