LANCE® TR-FRET Epigenetics Reagents

LANCE® TR-FRET Epigenetics Reagents
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 4.6/5.0 (1 review)

PerkinElmer, Inc.

LANCE® TR-FRET Epigenetics Reagents - Consistent, validated solutions for your most demanding epigenetics applications. Proven, cost-efficient LANCE Ultra reagents can be used to quantitate pept...read more

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Lihong Shi


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Member since 2011

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Takeda
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RATING: 4.6

  • 5 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 17 Jun 2011

LANCE® TR-FRET Epigenetics Reagents

"sensitive and easy to use."

Description

 

LANCE® TR-FRET Epigenetics Reagents - Consistent, validated solutions for your most demanding epigenetics applications.

Proven, cost-efficient LANCE Ultra reagents can be used to quantitate peptide modifications, detecting specific meythylation and acetylation states. No wash, homogenous LANCE Ultra reagents are HTS friendly – design your own epigenetics screening strategy for greatest efficiency.

LANCE Ultra time-resolved fluorescence resonance energy transfer (TR-FRET) assays use a proprietary europium chelate donor dye, together with ULight™, an innovative small molecular weight acceptor dye with a red-shifted fluorescent emission.

Epigenetic enzymatic assays are optimized using a biotinylated histone H3-derived peptide as substrate. The modified peptide is captured by the Eu-labeled antibody (Ab) and ULight-Streptavidin (SA) which bring the Eu donor and ULight acceptor dye molecules into close proximity. Upon irradiation at 320 or 340 nm, the energy from the Eu donor is transferred to the ULight acceptor dye which, in turn, generates light at 665 nm. The intensity of the light emission is proportional to the level of biotinylated substrate modification.

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