The Lambda ZAP-CMV vector allows production of libraries in a high-efficiency lambda vector. Libraries cloned into lambda vectors routinely produce greater than 4 times the primary library size as plasmid libraries. The Lambda ZAP II system combines the high efficiency of lambda library construction and the convenience of a plasmid system with improved blue white color selection.
High-efficiency lambda cloning with versatility of plasmid
in vivo excision of cloned fragments into phagemid vector
Expression of fusion proteins under control of lac promoter
Allows screening in lambda with DNA probe or in mammalian cells with functional screen
Create large, highly-representational libraries
Clone genes that are toxic in E.coli
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