Being able to analyze native samples and biological fluids is enhanced by the quick processing time of the LAESI DP-1000. Without the need of any upstream LC sample separation, samples are directly ablated and then analyzed. The precisely controlled stage moves with ultra-fast precision and control, enabling sample acquisition in seconds. Whether analyzing 96 or 384 well plates, the LAESI DP-1000 software allows for one a run per plate option for even faster data acquisition. The stage and control software acts as a temperature controlled autosampler, which allows for preprogramming and automatic sample analysis. For imaging of samples, various laser raster settings can be programmed for rapid biomolecular profiling.
The LAESI DP-1000 excels at providing both qualitative and quantitative data for two dimensional and 3D tissue imaging workflows. With virtually no sample preparation required, the LAESI DP-1000 elegantly maps the spatial distribution of any other desired biomolecule of interest in either two or three dimensions. Mapping is aided by the use of a high resolution, wide angle camera used for capturing pre- and post-analysis photos of the sample. In addition to the wide angle camera, a near-field camera is equipped for viewing and targeting the sample area of interest. In order to provide the accurate spatial resolution data, the LAESI DP-1000 uses an ultraprecise XYZ stage for supreme sample movement and control while scanning. Three dimensional imaging is made possible as the powerful onboard laser tunnels through thicker sections of tissues, while capturing data with every laser pulse. The laser tunneling eliminates the need for sample slicing and complicated sample alignment issues. Once the sample has been analyzed, the LAESI DP-1000 uses ProteaPlot™ imaging software to create publication quality contour plots showing the geographic distribution of molecules throughout the tissue.
Quick Workflow Integration and Flexibility
The LAESI DP-1000 conveniently integrates with different mass spectrometers, including single quad, triple quad, time of flight and HDMS systems from several manufactures. This integration capitalizes on high resolution, accurate mass and quantitative aspects of a coupled mass spec system. Fully adjustable height and cantilever movements of the LAESI DP-1000 easily adjust to fit various bench heights and mass spec set-ups. LAESI DP-1000 set-up and installation is intuitive, and the system can be easily removed for alternative upstream chromatography workflows using the same mass spectrometer.