Klenow Fill-In Kit by Agilent Technologies

Choose the Klenow Fill-In Kit to perform both partial fill-in reactions to prepare genomic DNA for insertion into the Lambda FIX II vector, and complete fill-in of a 5 overhang to generate blunt ends, to allow direct ligation. Or, use the kit to radioactively end label a 5 overhang with [alpha-³²P]dNTP. 

The Klenow Fill-in Kit contains Klenow fragment (125 U), dNTPs (separate 10 mM stocks), Klenow reaction buffer, predigested DNA, which can be used as a control, and sufficient material for 25 reactions.

Features:

• Perform partial fill-in reactions to prepare genomic DNA for insertion into the Lambda FIX II vector
• Perform a complete fill-in of a 5 overhang to generate blunt ends, to allow direct ligation
• Radioactively end label a 5 overhang with [alpha-³²P]dNTP
• Includes predigested DNA that can be used as a control

For Research Use Only. Not for use in diagnostic procedures.