KAPA HyperPlus Kits by Kapa Biosystems, Inc.
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Status:
Reviewer
Member since: 2020
Organization: ICMR-NIE
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- Ease of use
- After sales service
- Value for money
US Pulsenet also using this kit
Application Area: Library prep
"Easy to use"
Review date: 01 Dec 2023 | KAPA HyperPlus Kits
Status:
Advanced Reviewer Member since: 2007
Organization: Wellcome Trust Sanger Institute
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- Ease of use
- After sales service
- Value for money
Efficient and easy to use Illumina library prep kit without need for Covaris
Application Area:Illumina sequencing
"This is an easy to use streamlined kit for preparing Illumina sequencing libraries. One can make libraries in single tube or plates in a little over 2 hours. The chemistry is highly efficient (see Aigrain et al BMC Genomics 2016) allowing for lower input and/or fewer PCR cycles. Enzymatic cleavage appears non-biased."
Review date: 23 Dec 2016 | KAPA HyperPlus Kits
The KAPA HyperPlus kit provides a streamlined workflow that includes fragmentation and library preparation in a single tube.
Building on industry-leading library construction efficiencies, this integrated solution combines enzymatic fragmentation, similar in quality to mechanical shearing, with the speed and convenience of tagmentation-based workflows.
Benefits:
- DNA fragmentation and library prep in 2.5 hours
- Flexible DNA sample input from 1 ng – 1 µg
- Reduced bias and maximize sequence coverage
- PCR-free workflows
Features:
Tunable and Reproducible Fragmentation
- Adjust library insert sizes from 150 – 800 bp by varying fragmentation time
- Reproducible insert sizes across a range of GC content and DNA input amounts
Industry Leading Library Yields…
- Routinely achieve conversion rates >80% from ≥ 100 ng input
- Superior performance across a range of DNA input amounts
- High library yields enable PCR-free workflows from as little as 50 ng starting material
…Enable Superior Sequencing Results
- Higher conversion rates results in fewer amplification cycles and lower duplication rates
- Detect low-frequency mutations with high confidence due to greater library diversity and more uniform sequence coverage
Minimal Sequence Coverage Bias
- Lower sequence bias when compared to tagmentation and other enzymatic fragmentation methods
- Equivalent performance to mechanical shearing
- Less bias leads to more uniform sequencing coverage and reduced sequencing costs
Applications:
- Whole Genome Sequencing
- Whole Exome Sequencing
- Targeted Sequencing (custom panels)
- FFPE Sample Sequencing
Product Overview
