The novel one-tube chemistry, optimally formulated and evolved enzymes enable higher yields of adapter-ligated library and lower amplification bias. This translates to higher library diversity, lower duplication rates and more uniform coverage, particularly for FFPE and low-input samples.
Improve library yields and sequence quality:
- Higher library yields translate to greater molecular complexity
- Fewer cycles of amplification with KAPA HiFi DNA Polymerase results in lower duplication rates and improved coverage
Create high-quality libraries from FFPE samples:
- Generate high-quality libraries from 250 ng FFPE DNA or less
- Significantly lower duplication rates and higher coverage
Complete library construction in less than 3 hours:
- One-tube workflow with minimal cleanup steps reduces overall time, and minimizes hands-on time
- Sample-to-library in <2 hours for PCR-free workflows, or <3 hours with amplification
- Fewer handling steps lead to improved consistency and reproducibility
Improve performance with low-input samples:
- Generate more sequence-quality library molecules without increasing adapter-dimers
- Increase yields without inducing size bias