Status:
ReviewerMember since: 2015
Organization: Sanford Burnham Prebys Medical Discovery Institute
I am very satisfied with this instrument, which allows me to obtain the data we sought. A significant period of training and basic experimentation is mandatory to allow for the full use of the system in order to obtain valuable data.
Application Area: I use this equipment for animal imaging.
"I have used the IVIS imaging system for several years. It is used for the subcutaneous visualization and quantification of bioluminescent tumor cells in nude, NOD/SCID and NSG mice in the presence or absence of chemopreventive agents. "
The IVIS Spectrum is Revvity's market-leading preclinical imaging system. Like previous IVIS systems, it uses Xenogen’s novel patented optical imaging technology to facilitate non-invasive longitudinal monitoring of disease progression, cell trafficking, and gene expression patterns in living animals. The IVIS Spectrum's optimized set of high efficiency filters and spectral un-mixing algorithms lets you take full advantage of bioluminescent and fluorescent reporters across the blue to near infrared wavelength region. It also offers single-view 3D tomography for both fluorescent and bioluminescent reporters that can be analyzed in an anatomical context using our Digital Mouse Atlas.
For advanced fluorescence imaging, the IVIS Spectrum has the capability to use either trans-illumination (from the bottom) or epi-illumination (from the top) to illuminate in vivo fluorescent sources. 3D diffuse fluorescence tomography can be performed to determine source localization and concentration using the combination of structured light and trans illumination fluorescent images. The instrument is equipped with 10 narrow band excitation filters (30nm bandwidth) and 18 narrow band emission filters (20nm bandwidth) that assist in significantly reducing autofluorescence by the spectral scanning of filters and the use of spectral unmixing alogrithms. In addition, the spectral unmixing tools allow the researcher to separate signals from multiple fluorescent reporters within the same animal.