The iTaq™ universal SYBR® Green one-step kit is a fast and convenient solution for real-time PCR.
The powerful combination of iScript™ RNase H+ MMLV reverse transcriptase and hot-start iTaq DNA polymerase in one reaction, provides improved PCR efficiency, wider dynamic range, superior sensitivity and specificity, and inhibitor tolerance — even with cell lysates — without affecting performance.
iTaq™ Universal SYBR® Green One-Step Kit (172-5150) Features:
Enhanced efficiency, specificity, and sensitivity — our patented* inhibitor reducer prevents the interference of the RT enzyme, which leads to reduced sensitivity and efficiency, with the DNA polymerase during the replication of the cDNA.
Use any ROX dependent or independent real-time PCR systems — the universal reference dye in this supermix enables ROX normalization of qPCR data regardless of the ROX level requirements of the qPCR system.
Achieve superior gene expression results under various cycling conditions — our robust formulation delivers consistent performance in standard or fast cycling conditions, primer concentrations, sample and target types, and temperature ranges, allowing cost and time savings.
Obtain better results with qPCR assays — simplify your workflow and experimental design using our laboratory-validated PrimePCR™ assays.