iTaq™ Universal Probes One-Step Kit, 100 x 20 µl rxns, 1 ml by Bio-Rad
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Member since: 2016
Organization: University of North Carolina at Charlotte
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Easy to use for one go qPCR.
Application Area: One step amplification in qPCR
"This kit is highly effective in regards to one-step amplification and quantification of the samples. Pretty straightforward to work with and you can use for the cDNA preparation and gene quantification in one go if you have to get fast results and are handling a lot of samples. Good kit for the molecular biologists and for doing molecular biology experiments with ease."
Review date: 13 Nov 2020 | iTaq™ Universal Probes One-Step Kit, 100 x 20 µl rxns, 1 ml
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Use our combined iScript RNase H+ reverse transcriptase and antibody hot-start iTaq DNA polymerase probe kit formulation to complete your real-time reactions in one step — PCR efficiency is improved over a wide dynamic range and under various conditions.
- Advanced formulation for amplifying difficult target sequences and challenging samples
- Perform singleplex or multiplex reactions simultaneously
- Normalize qPCR data with ROX normalization dyes
Kit Components
- Reverse transcriptase — 50 µl
- Reaction mix — 1 ml
Instrument Compatibility
iTaq Universal Probes One-Step RT-qPCR Kit is compatible with Bio-Rad Real-Time PCR Detection Systems and commercially available systems listed below:
* BSA must be added according to instrument specifications
Applications and Uses of the iTaq Universal Probes One-Step Kit
- qPCR/real-time PCR
- Gene expression analysis
- Multiplexing
- Absolute quantification
- Mutation detection
- Pathogen detection
- Viral detection (load)
- Genetically modified organisms (GMO) characterization
- Genetic profiling
The iTaq Universal Probes One-Step Kit is also available in a format of 500 reactions ( 1725141 ).
Related Products
Bio-Rad has also developed one-step kits for SYBR® Green–specific applications .
