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iScript™ cDNA Synthesis Kit, 25 x 20 µl rxns by Bio-Rad
Manufacturer Bio-Rad | Model: 1708890 | Available Worldwide
25 x 20 µl reactions, reverse-transcription reagent kit, includes 5x iScript reaction mix, iScript reverse transcriptase, nuclease-free water
iScript™ cDNA Synthesis Kit, 25 x 20 µl rxns
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Average Rating: 4.9
4 Scientists have reviewed this product
Status:
Reviewer
Member since: 2019
Organization: Department Of Zoology
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- Ease of use
- After sales service
- Value for money
Desirable results are obtained by using this cDNA synthesis kit
Rating: 5.0
Application Area: Liver tissue samples
"The cDNA synthesis kit is totally fantastic and easy to use"
Review date: 17 Dec 2021 | iScript™ cDNA Synthesis Kit, 25 x 20 µl rxns
Status:
Advanced Reviewer Member since: 2011
Organization: MSK
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- Ease of use
- After sales service
- Value for money
Easy to use, great yield every time, painless
Rating: 4.7
Review date: 03 Nov 2017 | iScript™ cDNA Synthesis Kit, 25 x 20 µl rxns
Application Area:RT-PCT, qRT-PCR, etc
"Very easy to use, works every time, almost effortless. Kit shelf life is extremely long, never has a problem, best bang for the money!"
Review date: 03 Nov 2017 | iScript™ cDNA Synthesis Kit, 25 x 20 µl rxns
Status:
Expert Reviewer Member since: 2015
Organization: Postdoctoral Associate in Cambridge MA
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- Ease of use
- After sales service
- Value for money
Great for most cDNA for qPCR
Rating: 4.7
Review date: 15 Jan 2016 | iScript™ cDNA Synthesis Kit, 25 x 20 µl rxns
Application Area:cDNA for qPCR
"Great kit for the synthesis of cDNA, is very consistent. However, if you are utilizing a small amount of RNA this kit isn't that great. Would recommend a more sensitive RT."
Review date: 15 Jan 2016 | iScript™ cDNA Synthesis Kit, 25 x 20 µl rxns
Status:
Reviewer Member since: 2010
Organization: Ohio State University
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- Ease of use
- After sales service
- Value for money
Rating: 5.0
Review date: 29 Sep 2015 | iScript™ cDNA Synthesis Kit, 25 x 20 µl rxns
Review date: 29 Sep 2015 | iScript™ cDNA Synthesis Kit, 25 x 20 µl rxns
Use the iScript cDNA Synthesis Kit for first-strand cDNA synthesis for real-time qPCR. This two-tube kit can accommodate dilute RNA samples.
Key Features and Benefits
- Up to 15 µl of RNA in a 20 µl cDNA synthesis reaction
- Combined oligo(dT) and random primers provide unbiased representation of 5' and 3' regions
- Fast 26 min cDNA synthesis protocol
- Linear range of 1 µg–1 pg total input RNA using RNase H+ MMLV reverse transcriptase
See the iScript cDNA Synthesis Kit product page for more information and documentation.
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Online Resources
- qPCR/Real-Time PCR
- Genomics
