iScript™ Reverse Transcription Supermix for RT–qPCR (170-8840)
25 x 20 μl reactions, 100 μl of 5x supermix contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl2, stabilizers; 10 reactions of no–RT control supermix.
Use iScript reverse transcription supermix for RT-qPCR for fast, sensitive first-strand cDNA synthesis in gene expression analysis by real-time qPCR.
- 1-tube format with a preblended 5x supermix containing all the necessary components for reverse transcription except RNA template
- Fast 40-minute cDNA synthesis protocol
- 100 µl (enough for 25 x 20 µl reactions)
- 50 reactions of no-RT control supermix