The iScript one-step RT-PCR kit for probes is a highly sensitive solution for real-time quantitative RT-PCR on a broad range of thermal cyclers. cDNA synthesis and PCR amplification are conveniently carried out in the same tube, using the powerful combination of iScript RNase H+ reverse transcriptase and hot-start iTaq DNA polymerase.

The iScript one-step RT-PCR kit for probes is designed for use with all types of hybridization probes, including dual-labeled oligonucleotide probes, FRET probes, and molecular beacons.

The kit is designed to promote specific amplification over a broad dynamic range, while preventing the formation of primer-dimers and other nonspecific PCR artifacts. The magnesium concentration has been stringently optimized to support duplex RT-PCR reactions, as well as probe hybridization and hydrolysis.

• Accurate detection of 100 fg of input RNA
• Sensitive and specific reverse transcription using any hybridization probe chemistry
• Optimized reaction mixture
• Convenient one-tube reaction setup that minimizes handling and contamination risk