The iScript one-step RT-PCR kit for probes is a highly sensitive solution for real-time quantitative RT-PCR on a broad range of thermal cyclers. cDNA synthesis and PCR amplification are conveniently carried out in the same tube, using the powerful combination of iScript RNase H+ reverse transcriptase and hot-start iTaq DNA polymerase.
The iScript one-step RT-PCR kit for probes is designed for use with all types of hybridization probes, including dual-labeled oligonucleotide probes, FRET probes, and molecular beacons.
The kit is designed to promote specific amplification over a broad dynamic range, while preventing the formation of primer-dimers and other nonspecific PCR artifacts. The magnesium concentration has been stringently optimized to support duplex RT-PCR reactions, as well as probe hybridization and hydrolysis.
Accurate detection of 100 fg of input RNA
Sensitive and specific reverse transcription using any hybridization probe chemistry
Optimized reaction mixture
Convenient one-tube reaction setup that minimizes handling and contamination risk
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