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Expert ReviewerMembership Status
Member since: 2015
Organization: SUNY at Buffalo
Great for most cDNA for qPCR
Application Area: cDNA for qPCR
"Great kit for the synthesis of cDNA, is very consistent. However, if you are utilizing a small amount of RNA this kit isn't that great. Would recommend a more sensitive RT."
Member since: 2010
Organization: Ohio State University
It contains RNase H+ iScript reverse transcriptase for sensitivity, a premixed RNase inhibitor to prevent indiscriminate degradation of RNA template, and a unique blend of oligo(dT) and random primers. All components are optimized to yield sensitive, unbiased representation over a broad dynamic range, with minimal setup and reaction time.
RNase H+ MMLV reverse transcriptase delivers high sensitivity for real–time RT–PCR Optimized blend of oligo(dT) and random primers ensures complete and unbiased RNA sequence representation. Easy reaction assembly and streamlined protocol saves time and enhances reproducibility.