iScript™ Advanced cDNA Synthesis Kit for RT-qPCR (170-8842) by Bio-Rad
Manufacturer Bio-Rad
The iScript Advanced cDNA Synthesis Kit for RT-qPCR is an enhanced formulation that delivers increased data throughput from a single 20 µl reverse transcription (RT) reaction for real-time qPCR. This two-tube kit enables superior RT capacity of input RNA up to 7.5 µg.
Key Features and Benefits:
Increase qPCR data throughput and cost effectiveness from a single reaction — cDNA synthesized from higher input RNA amounts can be used for analyzing large numbers of target genes
Detect low-level t... Read more
iScript™ Advanced cDNA Synthesis Kit for RT-qPCR (170-8842)
Average Rating: 3.0
1 Scientist has reviewed this product
Status:
Reviewer
Member since: 2012
Organization: ASU
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- Ease of use
- After sales service
- Value for money
Rating: 3.0
Application Area: Neuroscience
"There is a lot of information about this synthesis product. The kit is useful when trying to obtain the best quality DNA."
Review date: 27 Nov 2012 | iScript™ Advanced cDNA Synthesis Kit for RT-qPCR (170-8842)
The iScript Advanced cDNA Synthesis Kit for RT-qPCR is an enhanced formulation that delivers increased data throughput from a single 20 µl reverse transcription (RT) reaction for real-time qPCR. This two-tube kit enables superior RT capacity of input RNA up to 7.5 µg.
Key Features and Benefits:
- Increase qPCR data throughput and cost effectiveness from a single reaction — cDNA synthesized from higher input RNA amounts can be used for analyzing large numbers of target genes
- Detect low-level target genes — uncompromised sensitivity even with less input RNA for when RNA sample is limited
- Reduce inter-assay variability and improve reproducibility — eliminate the need for multiple cDNA synthesis reactions
- Allow true representation of target mRNA levels — potent blend of RNase inhibitor prevents RNA degradation during reaction setup and RT
- Increase primer design flexibility and prevent 5' and 3' bias — optimum blend of oligo(dT) and random primers for superior RNA coverage
- Set up reactions with ease and obtain cDNA in less time — 2-tube kit generates cDNA in 35 min
