Status:
ReviewerMember since: 2016
Organization: NIEHS
Great antibodies, wouldn’t trade them for anything.
Application Area: Western blotting
"The IRDyes work quite nicely for standard western blotting applications. They are very strong/sensitive allowing you to stretch their usage quite a bit."
Status:
ReviewerMember since: 2016
Organization: University of North Carolina at Chapel Hill
Great choice in secondary antibody!
Application Area:Analyze phosphorylated proteins
"The IRDye antibodies are very easy to use. They give consistent and reliable results. Detection of my Western Blot bands is so simple when using this product."
Status:
ReviewerMember since: 2016
Organization: Temple University
The products are very good! Great services and instrument!
Application Area:Human Platelets and HEL cells
"1, Simply add a dilution amount of the expected antibody to the buffer and incubated for a specific time 2, The technical and sale support are helpful 3, The blocking buffer also worked well"
Status:
ReviewerMember since: 2016
Organization: The Scripps Research Institute
Trustable antibodies
Application Area:Western Blot
"These secondary antibodies work really well, I always get high quality and reproducible results, also, because you can use them really diluted and re-use them, they end up being quite cheap."
Status:
ReviewerMember since: 2015
Organization: SUNY Upstate Medical University
Western blots become much easier and quantifiable.
Application Area:Cortical tissue protein analysis
"The IR dye system is very efficient and reliable. It allows for multiplexing a protein of interest and a loading control protein or multiple proteins of interest. The concentrations used are very low, 1:20,000, and give great signal. One vial of IR dye will last a very long time. This blotting system also allows for easily quantified results. Representatives in my area have given demonstrations and have responded promptly to any question about the use of IR dyes."
Status:
ReviewerMember since: 2016
Organization: NIH
Sensitive Ab
Application Area:Western Blot
"The low Ab concentration generates strong signals. The antibody is sensitive and easy to use. It also last a very long time by storing in 4C fridge. It worth the money paid for it."
Status:
ReviewerMember since: 2016
Organization: The Scripps Research Institute
2 color is very useful for normalizing to control proteins
Application Area:Western blotting
"Great, strong, very stable signal and specificity; nice using on the Odyssey fluorescence detection system for 2 color analysis."
Status:
ReviewerMember since: 2013
Organization: Barrow Neurological Institute
Application Area:Western blotting the glycolytic enzyme, Hk2
"So far, we have gotten clear and sharp bands when analyzing our western blots using these secondary antibodies. It's an inconvenience to have to have the lights off whilst using the antibody, but it's a given and nothing more than inconvenience. I have never needed to actually talk to customer service."
Status:
ReviewerMember since: 2014
Organization: Johns Hopkins University
"IRDye® 800CW donkey anti-goat secondary antibody and other odessey secondary antibodies made me love western blot. They are easy to use and good value for the price."
Quality and performance of secondary antibodies is crucial for Western blotting, In-Cell Western™ Assays, immunohistochemistry, and many other applications.
IRDye and VRDye secondary antibodies are highly cross-adsorbed, making them suitable for multi-color detection. IRDye secondary antibodies have been optimized for use with the Odyssey® family of imaging systems and can be used on instruments with similar excitation and emission filters. VRDye secondary antibodies are optimized for microscopy, immunohistochemistry, and flow cytometry applications on instruments with suitable excitation and emission filters.
IRDye® and VRDye™ Secondary Antibodies:
IRDye®800CW
IRDye® 680RD
IRDye® 680LT
IRDye® 650
VRDye™ 549
VRDye™ 490
Manufacturer LI-COR Biosciences | Available Worldwide
4.6 / 5.0 | 9 reviews